Creative Biolabs provides anti-idiotypic antibody production service detecting FREE antibodies. We have extensive experience in developing high specificity and affinity anti-idiotypic antibodies (anti-ID Abs) detecting free antibodies to support your antibody drug development.
Creative Biolabs utilizes target antibodies in scFv forms or using peptides designed according to the sequences of the variable domains as targets to isolate anti-idiotypic antibodies from immunized phage display library. If variable regions of both the heavy- and light-chains contribute idiotypic determinants, the idiotype is likely to be a conformational determinant, then scFv form will be used. On the other hand, individual idiotypes may be located either in the heavy- or light-chains and contain the primary amino acid sequences of the variable regions. These idiotypes are more likely to be sequence-dependent, and linear antigenic determinants. In this situation peptides will be utilized as target proteins. Anti-ID antibodies can then be isolated by immunized phage display library technology.
These anti-ID antibodies targeting CDRs have the following properties:
• Paratope specific
Fig. 1 Binding mode of anti-idiotypic antibody detecting FREE antibody. Image shows anti-ID Fab antibody in purple and antibody in orange. (Turner 2014)
Please click here to see more custom Anti-Idiotypic Antibodies Production Services from Creative Biolabs to meet your specific requirements.