ProteOn™ System based Epitope Binning Service

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Epitope Binning Services by ProteOn Platform

A bin is related to one epitope of an antigen and can be considered as the antibodies' footprints on the antigen's surface. For epitope binning measurements, Creative Biolabs has extensive experience with ProteOn protein interaction array system. In particular, we are capable of epitope binning a large variety of antibody forms even in crude samples.

Creative Biolabs provides Surface Plasmon Resonance (SPR) services based on our unique ProteOn protein interaction array system. The ProteOn platform creates a six-by-six crisscrossing interaction array via six parallel injections over a sensor chip that swivels 90º from the direction of ligand to analyte. Thus, six analytes flow over six strips where spots that contain ligand (reaction spots) alternate with those that do not (interspots) for a total of 36 interaction surfaces and 42 reference surfaces.

Fig. 1 Classical sandwich assay on the ProteOn. (Abdiche et al. 2009)

Other optional monoclonal antibody epitope binning services:

Reference

Abdiche, Y. N., Malashock, D. S., Pinkerton, A. and Pons, J. (2009) 'Exploring blocking assays using Octet, ProteOn, and Biacore biosensors', Analytical biochemistry, 386(2), 172-180.

FAQ

What is the ProteOn protein interaction array system and how does it work?
The ProteOn protein interaction array system is a technology designed for the real-time analysis of protein interactions. It operates on the principle of surface plasmon resonance (SPR), which allows it to detect the binding and dissociation of molecules without the need for labels. This system features a unique 6x6 interaction array that can simultaneously analyze six ligands against six analytes, facilitating comprehensive multiplex analysis in a single experiment.
What types of samples can be analyzed using the ProteOn system?
The ProteOn system is versatile and can analyze a wide range of biological samples including proteins, peptides, small molecules, and even cells. Its robust design makes it suitable for use with crude or complex samples such as serum, plasma, or cell lysates, enabling direct interaction studies in a physiologically relevant context.
Can the ProteOn system be used for quantifying protein interactions?
ProteOn system is not only capable of identifying protein interactions but also quantifying them. It provides kinetic data such as association and dissociation rates (ka and kd), as well as equilibrium dissociation constants (KD), which are crucial for understanding the strength and specificity of interactions. This quantitative analysis is valuable in drug discovery and research, where precise interaction measurements are required.
What advantages does the ProteOn system offer over other protein interaction analysis technologies?
The ProteOn system offers several advantages, including the ability to perform real-time, label-free monitoring of interactions, which provides immediate insights into binding characteristics. Its unique 6x6 parallel processing capability allows for the simultaneous analysis of 36 interactions, significantly speeding up data acquisition and analysis compared to traditional single-channel SPR systems. This high-throughput capability makes it ideal for screening applications in drug discovery and basic research.