Creative Biolabs has developed ex vivo selection schemes using phage-displayed libraries. This can be used to discover peptide and protein ligands for macromolecules that are expressed in an organ- and tissue-specific manner. Our scientists commit to offer clients a reliable and robust ex vivo phage display service to meet each individual requirement.
The term of ex vivo phage display is a novel method using phage solutions to select the primary cell suspensions of organs and tissues or the cultured cells as targets. Compared with in vivo phage display, which phages are primarily exposed to the vascular endothelial cells, it is notable that ex vivo phage display is exposed to all cell types. Besides that, ex vivo phage display is higher throughput, which is able to screen a large amount of different phages by using material from one mouse. Moreover, ex vivo phage display also has lower background than in vivo phage display. In other words, ex vivo binding can be more discriminatory than in vivo homing. Therefore, the ex vivo phage display technology can be treated as a novel choice or combining with in vivo phage display technology to identify the most specific binding target.
The proprietary ex vivo phage display technology of Creative Biolabs is an improved biopanning technology for discovering peptide and protein ligands for organ- and tissue-specific macromolecules. Our scientists can generate high diversity phage display libraries with a great deal of candidate peptides and protein ligands. The peptides and ligands isolated by ex vivo phage display can distinguish between the different organs and tissues, and can be used as homing devices for therapeutic and imaging agents. Furthermore, they can be therapeutics themselves and provide tools for studying the underlying biology of processes such as lymphocyte homing, cancer metastasis, and the tropism of pathogenic microorganisms.
Creative Biolabs is a long-term expert in the field of phage display technology. With our most devoted scientists, fast delivery and efficient service are guaranteed. Meanwhile, as the chosen cooperation research partner of our global clients, Creative Biolabs is pleased to provide our professional skills to help customers achieve their research purpose and assist their project development.
Fig. 1 Combined application of ex vivo and in vivo phage display.