With years of experience in the area, scientists from Creative Biolabs now provides world-leading whole cell antigen presentation technology for anti-membrane protein antibody development. The core of this advanced technology is to present native membrane protein antigens on the surface of mammalian cells. This unique technology is contributing to the growing interest in exploring various therapeutic agents targeting membrane proteins.
Introduction of Whole Cell Antigen Presentation
Traditional anti-membrane protein antibody production methods involve a critical step of in vitro antigen production for animal immunization and antibody selection. However, the expression & purification process of membrane proteins is rather complicated and challenging due to their, hydrophobic nature, sophisticated structure, low natural abundance, unstability, toxicity, etc. After years of efforts and dedication, Creative Biolabs has launched the innovative whole cell antigen presentation technology. We have successfully developed multiple stably transfected cell lines of more than 200 membrane proteins (e.g. GPCR, ion channel, transporter). Notably, we also offer cutting-edge anti-membrane protein antibody production by dendritic cell antigen presentation service. By presenting antigens on mammalian cell plasma membranes, membrane proteins of interest can bypass all the drawbacks of in vitro antigen preparation, thus can be well applied in a variety of applications.
Advantages of Whole Cell Antigen Presentation
Compared with membrane protein antigens production in vitro, whole cell antigen presentation shows several appealing advantages. First, it is very difficult to prepare membrane protein antigens from plasma membranes into pure, homogenous and conformationally stable forms. In contrary, whole cell antigen presentation provides an easier alternative to keep membrane protein antigens in their native membrane environment with correct, stable, and functional foldings. No need for tedious purification, reconstitution steps. Moreover, detergents are usually a must to solubilize membrane protein targets from cell membranes, which have potential risks to disturb/impact original protein structure or modifications (especially long-chain detergents that tend to mask extracellular epitopes). In some cases, the functionality/conformation features cannot be restored, resulting in denatured products. While for whole cell presentation, no detergent or solubilization processes are needed.
Our technology involves immunization by injection of cultured cells stably over-expressing the antigens that avoid many drawbacks of in vitro antigen production. We have successfully generated many superior anti-membrane protein antibodies via this strategy.
Features of Our Service
In particular, we have successfully incorporated whole cell antigen presentation into following antibody discovery choices:
Creative Biolabs is professional membrane protein immunogen preparation of all kinds. We have successful experience with GPCR immunogens, ion channel immunogens, and transporter immunogens. Please feel free to contact us for more information.