Methylation-Specific Antibody Production Services

Creative Biolabs offers high-quality anti-methylated protein antibodies which are generated with our excellent High-Affi™ technology. The antibodies are capable of recognizing endogenous levels of lysine (mono-, di-, or tri-methylated) or arginine (mono-, asymmetric or symmetric di-methylated) residues. Also, the anti-methyl antibodies are site-specific and do not cross-react with acetylated proteins.

Protein methylation is a type of post-translational modification. Protein methylation typically takes place on arginine or lysine amino acid residues in the protein sequence. Arginine can be methylated once (monomethylated arginine) or twice, with either both methyl groups on one terminal nitrogen (asymmetric dimethylated arginine) or one on both nitrogens (symmetric dimethylated arginine) by peptidylarginine methyltransferases (PRMTs). Methylated arginine residues often occupy glycine-arginine rich (GAR) protein domains, such as RGG, RG, and RXR repeats. Lysine can be methylated once, twice or three times by lysine methyltransferases. Methylation is a reversible dynamic process, and the transferred methyl can remove by lysine demethylases (KDMs). Protein methylation has been most studied in the histones. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases (HMTs). Continuing evidence proves that non-histone proteins also occurs methylation, especially transcriptional regulation proteins like transcription factor p53 and estrogen receptor alpha. Modification of methylation on both histones and non-histone proteins play critical roles in gene transcription, protein synthesis, and cellular signal transduction processes. Methylation also acts with other post-translational modifications in regulating many aspects of protein function and life process. Evidence now suggests that the tumor suppressor protein p53 is subject to numerous modifications (e.g. phosphorylation, methylation, acetylation, ubiquitination, and neddylation). For example, monomethylation of K372 can facilitate acetylation of K373 and K382, and enhance the stability and activity of p53. Therefore, dysfunction of methylation impacts on a series of cellular events like cell cycle control, DNA repair, differentiation, apoptosis and causes tumorigenesis, neurodegenerative diseases, cardiovascular diseases and so on.

Methylation-Specific Antibody Production Services
Fig. 1 Types of arginine and lysine methylation.

The anti-methylated protein antibodies, which are purified by peptide affinity chromatography, are developed using High-Affi™ technology unique to Creative Biolabs. With the combination of phage display technology, the antibodies recognize various types of methylated residues and could be applied to the detection, quantization, and isolation of methylated proteins. Furthermore, a bunch of stunning results has been generated from immunoassays with our methylation-specific antibodies.

In addition to the methylation-specific antibody, Creative Biolabs also provides a comprehensive list of PTM-specific antibody production services of your choice.

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