By outsourcing in vitro mutagenesis projects to Creative Biolabs, your lab can profit from an extremely short turnaround time, even in demanding mutagenesis projects.
Mutagenesis is a fundamentally important DNA manipulation technique. It involves changing the base sequence of the DNA molecule, usually for the purposes of testing the effect of this change on gene or DNA function. Most site-directed mutagenesis is based on the PCR method, in which strand separation is accomplished by using a denaturing step to separate the complementary strands and in which efficient polymerization of the PCR primers is possible. In theory, the PCR method can be performed in the lab, but it requires considerable optimization, especially a staff skilled in both PCR and primer design. Often, the time and energy poured into in-lab PCR mutagenesis would be better spent elsewhere, such as on the lab's core creative work.
Fig.1 Protein Mutagenesis.
At Creative Biolabs, we guarantee top accuracy and unlimited mutant sites. Moreover, we offer comprehensive upstream and downstream services, including template sequencing, expression vector construction, and protein expression and purification.
In order to see how the physical properties of a particular residue influence the affinity of the protein-protein interaction, scientists usually perform site-directed mutagenesis of specific amino acid residues that are present at the protein-protein interface. Through this process, how specific protein-protein interactions are stabilized is also obtained exactly. Direct biochemical methods, genetic interaction methods, and computational inference methods are three different approaches for identifying mutagenized targets.
In silico prediction methods have increasingly been valuable and popular in molecular biology, especially in human genetics, for deleteriousness prediction to filter and prioritize huge amounts of DNA variation identified by sequencing human genomes. Large numbers of in silico methods for predicting the deleteriousness of a variant are available, among which similar or different sources of information or their combination are utilized in the prediction model. For example, sequence information, protein structure information and/or annotations from known databases or predictions results can be used to collect and calculate classification features.
Computational mutagenesis analysis can be performed on a large number of mutants, and with efficient algorithms and software, all possible mutants of a given protein can be analyzed. In most molecular dynamics’ software, standard and phosphorylated amino acids can be substituted site-specifically. Multiple mutations can be made by sequential mutagenesis within the software. Mutated molecules can be subsequently simulated using a few different strategies.
The proven expertise of Creative Biolabs’s team in the field of in vitro mutagenesis combined with our optimized technology allows us to deliver mutated and verified vectors (purified DNA and bacterial clones) within only a few days, thereby saving scientists' valuable time. In addition, we can provide a series of mutagenesis services together with Creative Biomart, including Site-Directed Mutagenesis, Site-Saturation Mutagenesis as well as Random Substitutions.
For more information, please feel free to contact us for more details.