By outsourcing in vitro mutagenesis projects to Creative Biolabs, your lab can profit from an extremely short turnaround time, even in demanding mutagenesis projects.
Mutagenesis is a fundamentally important DNA manipulation technique. It involves changing the base sequence of the DNA molecule, usually for the purposes of testing the effect of this change on gene or DNA function. Most site-directed mutagenesis is based on the PCR method, in which strand separation is accomplished by using a denaturing step to separate the complementary strands and in which efficient polymerization of the PCR primers is possible. In theory, the PCR method can be performed in the lab, but it requires considerable optimization, necessitates a staff skilled in both PCR and primer design. Often, the time and energy poured into in-lab PCR mutagenesis would be better spent elsewhere, such as on the lab's core creative work.
At Creative Biolabs, we guarantee top accuracy and unlimited sites. Moreover, we offer comprehensive upstream and downstream services, including template sequencing, expression vector construction, and protein expression and purification. The proven expertise of Creative Biolabs team in the field of in vitro mutagenesis combined with our optimized technology allows us to deliver mutated and verified vectors (purified DNA and bacterial clones) within only a few days, thereby saving scientists' valuable time.