Creative Biolabs offers a customized service for native rat antibody development to produce rat monoclonal antibodies from peptide or protein antigens.
Over the years, rat, just like its rodent brother, mouse, plays a key role in many experimental studies. Together with mice and guinea pigs, rats belong to the largest and most diversified order of mammals, the Rodentia. Since 18th century, the first time domestic albino brown rats were used for purely scientific purposes, a great deal of advanced understanding associate with genetics, therapeutics, pathology and other topics, that have provided a great benefit for the health and well-being of humankind, must be owed to the sacrifice of countless laboratory rats. Currently, rat is the second most frequently used experimental animal host, after the mouse, for the monoclonal antibody production. Same as human and mouse, rat has five antibody isotypes (IgA, IgD, IgE, IgG and IgM). Furthermore, the canonical protein sequence comparison between cognate mouse and rat shows a high similarity between their immunoglobulin constant regions.
Compared with mouse antibodies, rat monoclonal antibodies offer several advantages, including the reduced antigen requirement, the availability of using mouse antigens and greater lymphocyte yields. Moreover, rat antibodies pair better than that of mouse in sandwich assays: there is no cross reaction of available anti-mouse secondary antibody binding to the capture rat antibody.
Using our exclusive antigen-specific B lymphocytes cytometry technology, Creative Biolabs has built the unique Native™ Antibody Discovery Platform. This proprietary platform can serve our clients with high-throughput discovery of native rat antibodies from antigen preparation, immunization and antibody purification. More notably, the entire candidate antibody pool is isolated as in vivo native paired heavy- and light-chains, resulting in tremendously higher specificity and affinity than any other traditional antibody production technology.
Creative Biolabs devotes to work together with each client throughout every step of the project to deliver research goal. Our experienced scientists are confident in generating antibodies that recognize not only the immunizing peptide fragment but also the native target protein so as to ensure its functions in client’s application in the most satisfactory manner.