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Recombinant Mouse Antibody scFv Fragment is bind to Human FASLG, expressed in E. coli
Recombinant soluble active extracellular domain of Human FAS-Ligand.
Suitable for use in ELISA, WB, Neut and most other immunological methods.
Tested positive against native antigen.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Store it under sterile conditions at -20°C upon receiving. Recommend to pack the protein into smaller quantities for optimal storage.
Flow Cyt: Use at a concnetration of 1-2 μg/test. Determined by flow cytometry using cultured KFL9 cells (human chronic myelogenous leukemia cell line K562, transfected with human Fas ligand). When assayed by flow cytometric analysis, using the antibody at working concentration to stain 1 x 106 to 2 x 106 cells/ 0.1ml/ test, fluorescence intensity is observed similar to that obtained with saturating antibody levels. The percentaggede population positive is also at the maximum percentaggede positive, using saturating antibody levels.IP: Use at an assay dependent dilution. Observed to bind an unknown 55 kDa protein (antigen = 33 kDa) during immunoprecipitation.