Recombinant Human Endostatin

Cat

CRP0868

Product Overview

Recombinant Human Endostatin produced in Pichia P. is a single glycosylated polypeptide having a total molecular mass of 20 kDa.

Specificity

Fully biologically active when compared to standard. The activity calculated by ECE migration inhibition was found to be 50,000IU/mg.

Endotoxin

Less than 1EU/μg of rHuEndostatin as determined by LAL method.

Purity

>96% by SDS-PAGE and HPLC analyses.

Storage

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

Endostatin monomer, basic amino acid residues shown in red1KOE.

Antigen Description

Endostatin has been identified as a C-terminal fragment of Collagen type 18, a recently identified member of a family of collagen-like proteins referred to as multiplexin familyEndostatin specifically inhibits proliferation of endothelial cells although it does not affect the proliferation of EOMA cells. Endostatin also potently inhibits angiogenesis and tumor growth. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes. Recombinant Endosatin expressed in yeast causes G1 arrest of endothelial cells, and endostatin treatment results in apoptosis of HUVE and HMVE cells.

Function

binding, extracellular matrix structural constituent, metal ion binding, protein binding, structural molecular activity, zinc ion binding.

Gene ID

80781

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