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Recombinant Human HBV core protein produced in E. coli has a molecular mass of 16823 Da.
Immunoreactive with sera HBV-infected individuals.
less than 10EU/mg as determined by LAL method
≥95% as determined by SDS-PAGE.
HBV Core protein is shipped at ambient temperature. Upon arrival, Store at -20°C . Five years frozen. One month in solution at room temperature.
Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA (pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.