Recombinant Human Interferon-alpha 2b

Cat

CRP0813

Product Overview

Recombinant Human Interferon-alpha 2b produced in E. coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19400 Dalton. The Interferon-alpha 2b gene was obtained from human leukocytes.

Specificity

The specific activity as determined in a viral resistance assay using VSV- WISH cells was found to be greater than 1.0 x 10⁸IU/ mg.

Endotoxin

Less than 0.3ng/ug (0.3IEU/ug) determined by LAL test.

Purity

>95% as determined by SEC-HPLC and SDS-PAGE .

Storage

Lyophilized rHuIFN-alpha 2b although stable at room temperature for 3 weeks, should be stored desiccated below -18^oC. Upon reconstitution rHuIFN-alpha 2b should be stored at 4^oC between 2-7 days and for future use below -18^oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.

Reconstitution

It is recommended to reconstitute the lyophilized rHuIFN-alpha2b in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Antigen Description

At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.

Function

interferon-alpha/beta receptor binding

Gene ID

3440

Synonyms

IFNA, INFA2, MGC125764, MGC125765; Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN-α 2a, Interferon alpha-A, LeIF A, Interferon alpha-2; Interferon alpha-2 Precursor

Download Datasheet:

CRP0892

Recombinant Human Osteoprotegerin/Fc Chimera

CRP08127

Recombinant Hepatitis B Surface Antigen preS2

CRP0824

Recombinant Human Interleukin-16

CRP0808

Recombinant Human Granulocyte Macrophage Colony Stimulating Factor

CRP0857

Recombinant Human PEX

CRP08134

Recombinant Human Cell Division Cycle 34 Homolog

CRP08133

Recombinant Rat Tropic 1808

CRP0850

Recombinant Fire Fly Luciferase

CRP0891

Recombinant Human Epidermal Growth Factor Receptor 3 Fragment

CRP08131

Recombinant Human Cyclin-Dependent Kinase Inhibitor 2A