Recombinant Human Interleukin-8(3-79)

Cat

CRP0823

Product Overview

Recombinant Human IL-8 (3-79) produced in E. coli is a single, non-glycosylated polypeptide chain containing 77 amino acids and having a molecular mass of 8904 Dalton.

Specificity

rHuIL-8 /77 is fully biologically active when compared to standard. The ED50 as determined by its chemotaxis of hCXCR-2 transfected mouse BaF/3 cells is less than 2 ng/ml, corresponding to a Specific Activity of 5.0 x 10⁵IU/ mg.

Endotoxin

Less than 0.1ng/ug (1 IEU/ug) determined by LAL test.

Purity

>95% as determined by RP-HPLC and SDS-PAGE.

Storage

Lyophilized rHuIL-8 /77 although stable at room temperature for 3 weeks, should be stored desiccated below -18^oC. Upon reconstitution rHuIL-8 /77 should be stored at 4^oC between 2-7 days and for future use below -18^oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.

Reconstitution

It is recommended to reconstitute the lyophilized rHuIL-8 /77 in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

PDB rendering based on 1IL8.

Antigen Description

Interleukin-8 (IL-8) belongs to the neutrophil-specific CXC family of chemokines. It is one of the initial cytokines released from a variety of cell types, including T cells, endothelial cells and fibroblasts, in response to an inflammatory stimulus and acts by recruiting neutrophils, T-cells and basophils to the site of inflammation. Elevated Interleukin-8 levels are associated with the onset of a variety of disease states.

Function

chemokine activity; interleukin-8 receptor binding; protein binding

Gene ID

3576

Synonyms

3-10C, AMCF-I, CXCL8, GCP-1, Monocyte-derived neutrophil chemotactic factor, MDNCF, T-cell chemotactic factor, Neutrophil-activating protein 1, NAP-1, Protein 3-10C, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil-activating peptide, MONAP, Emoctakin, K60, NAF, LECT, LUCT, LYNAP, SCYB8, TSG-1, b-ENAP;GCP1; IL-8; NAP1; C-X-C motif chemokine 8;Interleukin-8 precursor ;beta-thromboglobulin-like protein; chemokine (C-X-C motif) ligand 8;lymphocyte-derived neutrophil-activating factor ;small inducible cytokine subfamily B member 8

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