Recombinant Human Keratinocye Growth Factor-1

Cat

CRP0882

Product Overview

Recombinant Human Keratinocye Growth Factor-1 produced in E. coli is a single non-glycosylated polypeptide chain containing 164 amino acids and having a molecular mass of approximately 19.0 kDa.

Specificity

The biological activity was determined by the does-dependent stimulation of thymidine uptake by BaF3 cells expressing KGF receptors yielding an ED50 <10ng/ml.

Endotoxin

Less than 1EU/mg of rHuKGF-1 as determined by LAL method.

Purity

>96% by SDS-PAGE and HPLC analyses.

Storage

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

PDB rendering based on 1qqk.

Antigen Description

Keratinocyte Growth Factor-1 (KGF-1/FGF-7) is one of 23 known members of the FGF family. All FGFs have two conserved cysteine residues and share 30 - 50% sequence identity at the amino acid level. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of variety of tissues, by promoting cellular proliferation and differentiation. KGF-1/FG-7 is a mitogen factor specific for epithelial cells and keratinocytes and signals through FGFR 2b. KGF-1/FGF-7 plays a role in kidney and lung development, angiogenesis, and wound healing.

Function

growth factor activity

Gene ID

2252

Synonyms

KGF; HBGF-7; FGF-7; Fibroblast growth factor 7; keratinocyte growth factor; Keratinocyte growth factor precursor; fibroblast growth factor 7 (keratinocyte growth factor)

Download Datasheet:

CRP0859

Recombinant Yeast Sup 35 (NM)

CRP08120

Recombinant Streptokinase

CRP0898

Recombinant Rat Interferon-γ

CRP0815

Recombinant Human HSA-Interferon alpha-2b

CRP0878

Recombinant Human Interleukin-1 alpha

CRP0840

Recombinant Human Angiostatin

CRP0849

Recombinant Human C-Terminal Fragment of Kinesin Heavy Chain

CRP0807

Recombinant Human Vascular Endothelial Growth Inhibitor

CRP08100

Recombinant Murine Vascular endothelial growth factor 120

CRP0871

Recombinant Bovine Fibroblast Growth Factor-basic