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Recombinant Human Long Arg3 Insulin-like Growth Factor-I (CRP0805)

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DESCRIPTION CATALOG # SIZE PRICE
Recombinant Human Long Arg3 Insulin-like Growth Factor-I CRP0805 1mg Please Inquiry

PRODUCT INFORMATION

  • Product Overview
  • Recombinant Human Long-R3-IGF-I produced in E. coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9.111 kDa.
  • Specificity
  • The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of 1.0 x 106 IU/mg. For most in-vitro applications, Long-R3-IGF-I exerts its biological activity in the concentration range of 0.2-20 ng/ml.
  • Endotoxin
  • Less than 0.1ng/ug (1IEU/ug) of Long-R3-IGF-I.
  • Purity
  • >95% by SDS-PAGE and RP-HPLC.
  • Storage
  • Lyophilized Long-R3-IGF-I although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Long-R3-IGF-I should be stored at 4oC between 2-7 days and for future use below -18oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.
  • Reconstitution
  • It is recommended to reconstitute the lyophilized Long-R3-IGF-I in 500mM acetic acid not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
  • Recombinant Human Long Arg3 Insulin-like Growth Factor-I
    PDB rendering based on 1bqt.

BACKGROUND

  • Antigen Description
  • Human Long Arg3 Insulin-like Growth Factor-I (Long-R3-IGF-I) is an 83 amino acid analog of human IGF-I comprising the complete human IGF-I sequence with the substitution of an Arg for the Glu at position 3 (hence R3), and a 13 amino acid extension peptide at the N-terminus. Scientists have engineered this analog with the express purpose of increasing biological activity. Human Long-R3-IGF-I is significantly more potent than human IGF-I in vitro. The enhanced potency is due to the markedly decreased binding of human Long-R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs.
  • Function
  • Growth factor activity, Hormone activity, Insulin-like growth factor receptor binding, Prothoracicotrophic hormone activity
  • Gene ID
  • 3479
  • Synonyms
  • IBP1, IGF-IA, IGF-IB, IGFI, MGF, Somatomedin-C; Insulin-like growth factor IA precursor; Insulin-like growth factor IB precursor; Mechano growth factor; insulin-like growth factor 1

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