Recombinant Human Tumor necrosis factor alpha

Cat

CRP0806

Product Overview

Tumor necrosis factor alpha-1a or TNFalpha-1a is a non-glycosylated cytokine produced from E. coli using rDNA technology. The protein consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. The individual subunits have a relative molecular mass each of 17.484 kDa.

Specificity

The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.03ng/ml, corresponding to a Specific Activity of 3.0 x 10⁷ IU/mg.

Endotoxin

Less than 0.01ng/ug (0.01IEU/ug) determined by LAL test.

Purity

>95% by SDS-PAGE and SEC-HPLC .

Storage

Lyophilized samples are stable for up to twelve months from date of receipt at -20^oC to -70^oC. Aliquot to avoid repeated freeze-thaw cycles.

Reconstitution

It is recommended to reconstitute the lyophilized TNF alpha-1a in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

PDB rendering based on 1TNF.

Antigen Description

TNF-alpha is a homotrimer with a subunit molecular mass of 17 kDa and that it plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases; and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF was found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn’s disease, and rheumatoid arthritis as well as graft-versus-host disease.

Function

Cytokine activity, Identical protein binding, Tumor necrosis factor receptor binding

Gene ID

7124

Synonyms

DIF; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor precursor; TNF-alpha; TNF-α; Tumor necrosis factor ligand superfamily member 2; Cachectin

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