Introduction of ABCB11
ABCB11 encoded by ABCB11 gene, also known as BSEP, is a member of the superfamily of ATP-binding cassette (ABC) transporters which transport various molecules across extra- and intracellular membranes. There are seven subfamilies in ABC superfamily named as ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White, respectively. BSEP possesses 12 transmembrane domains and belongs to MDR/TAP subfamily. It is most expressed in the canalicular membrane of hepatocytes.
|Basic Information of ABCB11|
|Protein Name||Bile salt export pump|
|Aliases||BSEP, PGY4, SPGP, ABC16, BRIC2, PFIC2, PFIC-2|
|Organism||Homo sapiens (Human)|
Function of ABCB11 Membrane Protein
ABCB11 is an ATP-dependent transporter of conjugated bile acids such as taurine-amidated and acyl-glucuronides. It is also reported as a high-affinity bile salt transporter such as glycochenodeoxycholate, glycocholate and taurochenodeoxycholate. ABCB11 is a major regulator of bile formation and bile flow, which plays an essential role in maintaining normal liver functions. The lacking ABCB11 function can decrease biliary bile salt secretion and bile flow, as well as the accumulation of the bile salts in hepatocytes, resulting in hepatotoxicity or cholestasis in humans. It has been revealed that mutations of ABCB11 have an association with the progression of cholestatic liver disease such as progressive familial intrahepatic cholestasis type 2 (PFIC2). And PFIC2 may increase the susceptibility of hepatocellular carcinoma in early life. Moreover, the variants in ABCB11 have been also revealed a role in the progression of anti-tuberculosis drug-induced cholestatic liver injury. Besides, ABCB11 also affect drug absorption by regulating the production of bile salt. It can bind to biliary cholesterol and phospholipids forming the micelles which promote drugs solubilization and absorption by the intestine.
Fig.1 Hypothetical mechanism of canalicular lipid excretion. (Elferink, 2007)
Application of ABCB11 Membrane Protein in Literature
The study shows nine major genomic variations in ATP8B1, ABCB11 and ABCB4 genes associated with one-third of Indian children with progressive familial intrahepatic cholestasis (PFIC).
The study indicates that the variant c.386G>A (p.C129Y) in ABCB11 is associated with progression of familial intrahepatic cholestasis type 2 with an impaired biliary excretion from hepatocytes and the inexistence of canalicular BSEP expression in liver histological assessments.
The study reveals that the Egyptian patients with chronic hepatitis C genotype 4 who carry CC genotype of ABCB11 SNP 1331T > C and present high plasma bile acid levels are linked to advanced hepatic fibrosis.
The study identifies five rare synonymous or intronic variants in ABCB11 heterozygotes, which are involved in the progression of intrahepatic cholestasis with low γ-glutamyltransferase.
The study expands known variants in ABCB4 and ABCB11 and identifies the roles in intrahepatic cholestasis of pregnancy (ICP) for mutations in ATP8B1 and ABCC2.
ABCB11 Preparation Options
To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-ABCB11 antibody development services.
As a forward-looking research institute as well as a leading custom service provider in the field of membrane protein, Creative Biolabs has won good reputation among our worldwide customers for successfully accomplishing numerous challenging projects including generation of many functional membrane proteins. Please feel free to contact us for more information.
All listed customized services & products are for research use only, not intended for pharmaceutical, diagnostic, therapeutic or any in vivo human use.