Introduction of ABCB5
ABCB5 encoded by ABCB5 gene, also known as P-glycoprotein ABCB5, is an integral membrane protein which belongs to the superfamily of ATP-binding cassette (ABC) transporters. The ABC transporters can transport various molecules across extra- and intracellular membranes in an ATP-dependent manner. And the substrates of these proteins are diverse ranging from small ions, sugars, and peptides to more complex organic molecules. ABCB5 is a drug efflux transporter that exists in a number of stem cells.
|Basic Information of ABCB5|
|Protein Name||ATP-binding cassette sub-family B member 5|
|Aliases||ABCB5beta, EST422562, ABCB5alpha|
|Organism||Homo sapiens (Human)|
Function of ABCB5 Membrane Protein
ABCB5 is a drug efflux transporter that plays a role in drug resistance. ABCB5 has been identified to regulate doxorubicin resistance in human malignant melanoma. In colorectal cancer, ABCB5 was shown to serve as a mediator of chemoresistance against 5-FU. The ABCB5 gene silence regulated by shRNA can decrease chemoresistance against 5-FU in human colorectal cancer cells. Furthermore, ABCB5 has been identified as an important regulator for tumor growth, invasion, and metastasis. In melanoma, ABCB5 can promote melanoma metastasis by blocking p65 ubiquitination to facilitate p65 protein stability. In breast cancer, ABCB5 significantly promote migration and invasion of breast cells as well as epithelial-mesenchymal transition through regulating downstream ZEB1. An increased expression of ABCB5 was observed in ocular surface squamous neoplasia. These results also suggest that ABCB5 can act as a novel potential therapeutic target for tumor. In addition, ABCB5 is necessary for corneal development and repair. The lack of ABCB5 functions leads to defective limbal stem cells (LSCs). This also suggests that ABCB5 may be a potential therapeutic target for corneal disease.
Fig.1 Immunofluorescence double labeling of ABCB5 with CD44 and survival curves of oral squamous cell carcinoma (OSCC) patients measured by ABCB5 expression. (Grimm, 2012)
Application of ABCB5 Membrane Protein in Literature
The genotyping study shows an association of four-allele combination I-D-T-G, (MS4A14/SLC2A7/PSG9/ABCB5) with a decreased susceptibility for recurrent pregnancy loss (RPL). Moreover, MS4A14 DI+II, SLC2A DI+II and ABCB5 CG+GG genotypes are associated with more than 12 sec prothrombin time and with RPL susceptibility.
The study shows that ABCB5 may not be a major regulator in BRAF inhibitor-resistant melanoma cell lines and p-ERK inhibition may exert essential roles in BRAF resistance in SK-MEL-28PLXr and A2058PLXr two melanoma cell lines.
The study shows a very important role of ABCB5 in colorectal cancer (CRC) invasiveness, suggesting ABCB5 blockade may act as a novel therapeutic drug in CRC.
The study reveals that the differential expression pattern of ABCC11 and ABCB5 genes may be associated with the incidence of multifocal/multicentric breast cancer (MMBS).
The study indicates that ABCB5 is involved in the regulation of melanoma metastasis and can be regarded as a promising target for melanoma treatment.
ABCB5 Preparation Options
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