Anti-OmpA Antibody Development

As the leading service provider in the world, Creative Biolabs is professional in the field of therapeutic and diagnostic antibodies development. Based on our advanced platform and extensive experience, now Creative Biolabs provides comprehensive customized antibody development services for Escherichia coli (E.coli) outer membrane protein A (OmpA) to help our clients make progress in their projects. We can provide the antibody development service against different protein biomarkers of E.coli, which including but not limited to OmpA. We are confident in providing unique antibody development service to meet every customer's requirements.

Escherichia coli

E.coli is a kind of Gram-negative rod-shaped, coliform bacterium of the genus Escherichia, which is generally found in the lower intestine of endotherms. Most E. coli strains are harmless and are part of the normal microbiota of the gut, they secrete vitamin K2 and prevent colonization of other intestine pathogenic bacteria are beneficial for hosts. However, some serotypes can cause serious food poisoning in their hosts due to food contamination. Pathogenic strains cause disease mainly via the fecal-oral transmission.

Escherichia coli is a rod-shaped Gram-negative.Fig.1 E.coli is a rod-shaped Gram-negative.

OmpA

E.coli Outer membrane protein A (OmpA) is a major heat-modifiable OMP, its molecular mass range from 28 to 36 kDa depending on the temperature and conditions to which it is subjected. The structure of OmpA is characterized by an N-terminal domain that forms an eight-stranded, anti-parallel β barrel, which is embedded in the outer membrane. It anchors to peptidoglycan noncovalently that is responsible for porin activity. The eight strands are linked at the outer membrane surface by four long loops and three short periplasmic turns of the molecule. OmpA has both structural and ion-permeable porin roles that guarantee bacterial survival during osmotic stress. Its expression is closely associated with its type 1 fimbriae expression. In addition, OmpA serves as a pathogen-associated molecular pattern (PAMP) that interacts with Toll-like receptor (TLR) 2 activating host immune responses. OmpA interacts with OMP TraN functionally and is important for conjugation.

The atomistic simulations of outer membrane protein OmpA with peptidoglycan. The structures reveal a conserved binding mode and a potential role of OmpA dimerization in maintaining the integrity of the bacterial cell wall.Fig.2 The atomistic simulations of outer membrane protein OmpA with peptidoglycan. The structures reveal a conserved binding mode and a potential role of OmpA dimerization in maintaining the integrity of the bacterial cell wall (Samsudin, 2016).

Antibodies against OmpA for AACs

Antibody-antibiotic Conjugate (AAC) that use the specific monoclonal antibody targeting OmpA enable the delivery of antibiotic exclusively to the infection site accurately, thereby reducing collateral damage to the normal tissues. Anti-OmpA AAC typically consists of two components, a monoclonal antibody targeting OmpA residues, and a cytotoxic antibiotic. Upon OmpA mAb binding, AAC is internalized via receptor-mediated endocytosis and the antibiotic payload is released to accomplish its cytotoxic effects.

Creative Biolabs can tailor high-quality antibody development services against OmpA and provide comprehensive strategies regarding AAC development. If you are interested in our service, please do not hesitate to contact us for more information and a detailed quote.

Reference

  1. Samsudin, F.; et al. (2016). “OmpA: a flexible clamp for bacterial cell wall attachment”. Structure, 24(12), 2227-2235.

For Research Use Only. NOT FOR CLINICAL USE.


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Antibody Discovery for Bacterial Infection: Antibody-antibiotic Conjugate (AAC): Services:
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