AOC for Electrochemical Proximity Assay (ECPA)

Antibody-oligonucleotide conjugates (AOCs) have been developed to improve efficient and precise detection, which are widely used in various biologic applications, for example, biomarker discovery, clinical diagnostics and therapeutics, protein interaction, network mapping... Creative Biolabs has rich experience in the field of bioconjugation, and we offer custom AOCs development service which permits highly sensitive immunoassay such as electrochemical proximity assay (ECPA). Our services will meet every client’s specific requirements and our experienced experts will work closely with you to make your programs a success.

AOC Used for Electrochemical Proximity Assay (ECPA)

ECPA is a simple electrochemical form, which is based on the combination of the concept of proximity assay and electrochemical detection. ECPA produces a direct readout, which is suitable for high sensitivity (low femtomolar (FM) range) and selective quantification of various protein targets. ECPA consists of binding molecules (initially aptamers) and oligonucleotide (ON)-tags. Upon the formation of a cooperative complex with a protein target causing a redox-active moiety such as methylene blue closer to a gold electrode, it can produce a redox current. The current can be quantified with high precision and directly depends on the concentration of the target.

AOC used in the ECPA mainly utilizes the proximity effect of two AOC probes bound to a protein target to move the electrochemical active label-methylene blue (MB) closer to the gold electrode with preassembled thiolated DNA/competitor monolayer. In the presence of a protein target, the redox current in ECPA is quantitatively analyzed by square wave voltammetry (SWV). It is found that the redox current is directly dependent on the concentration of the target. ECPA can be used for any protein with available antibody pairs.

Fig.1 Mode of action of ECPA for protein detection. (Dovgan, 2019)

Featured AOC Development Services at Creative Biolabs

Equipped with a comprehensive and state-of-the-art technical platform, Creative Biolabs offers proficient and fast-delivery AOCs development services. With over a decade of rich experience in antibody development and bioconjugation, we provide personalized solutions for joining different biomolecules through either chemical or biological approaches to form a novel complex. Some of our most popular conjugation and featured services include antibody-drug conjugates (ADCs), immunogen conjugation, antibody-enzyme conjugation, and antibody-fluorescent dye conjugations.

Features of Our Services

• Effective conjugation performed by professional scientists
• Advanced technology platform for fast product delivery
• Stable and effective conjugates for a specific application
• Cost-effective and timely after-sales technical support

If you are interested in our AOCs preparation services and want to know more information, please feel free to contact us. We will get in touch with you as soon as possible.

Reference

1. Dovgan, I.; et al. Antibody-oligonucleotide conjugates as therapeutic, imaging, and detection agents. Bioconjugate chemistry. 2019, 30(10): 2483-2501.

For Research Use Only. NOT FOR CLINICAL USE.