AOC for Super-Resolution Microscopy

Antibody-oligonucleotide conjugate (AOC) is a novel format composed of antibody conjugated to an oligonucleotide (ON), which is widely used in numerous fields such as targeted therapy, detection, and diagnosis. However, the development of an effective AOC is critical, thus, a suitable conjugation method to generate a high-quality conjugate is important.

At Creative Biolabs, we are well-trained professionals in various conjugations such as antibody-oligonucleotides (DNA and RNA) conjugation. Our unique conjugation technology is the most efficient and robust method in the field and allows the conjugation of single- and double-stranded oligonucleotides to the antibody. Therefore, our well-established protocols allow us to provide reliable custom oligonucleotide conjugation services to meet your special needs.

Fig.1 Simplified schematic representation of AOC and typical distribution of species with different DoC in a lysine-conjugated AOC. (Dovgan, 2019)

AOC Used for Super-Resolution Microscopy

Super-resolution microscopy, also known as stochastic nanoscopy, is developed to overcome the resolution limit of conventional light microscopy. This technology allows images to have resolutions higher than those imposed by the diffraction limit, which is due to the diffraction of light.

AOC can be used in super-resolution microscopy, such as STORM (Stochastic Optical Reconstruction Microscopy), STED (Stimulated Emission Depletion) microscopy, and SIM (Structured Illumination Microscopy). In this technique, the labeling strands are specifically designed to form stable binding during image acquisition, thus allowing the sequential imaging of different antibody targets. Significant in situ signal amplification can be achieved by a more elaborated oligonucleotide design, which is used as a docking site for multiple fluorophore-bearing DNA imager strands, to realize controllable multiplexed signal amplification. This method can be used to achieve the super-resolution imaging of various biological systems and to measure the precise distributions of different cellular targets.

Fig.2 AOCs can be used in super-resolution microscopy detection. (Cremers, 2019)

Featured AOC Development Services at Creative Biolabs

To perform successful bioconjugates, usually, it requires sufficient knowledge of the reactive groups, reagent types, and functional groups present on the biomolecules, as well as an understanding of how modification of a biomolecule can potentially affect its activity. Creative Biolabs has rich knowledge and extensive experience in bioconjugation chemistry, and we are ready to assist you step by step from the design to the final implementation and manufacturing to fit your specific need. We are committed to antibody development and antibody-related bioconjugation for many years, with a strong focus on a wide range of modifications.

If you are interested in our AOCs development services, please contact us for further information or an informal discussion of your required specifications.

References

1. Dovgan, I.; et al. Antibody-oligonucleotide conjugates as therapeutic, imaging, and detection agents. Bioconjugate chemistry. 2019, 30(10): 2483-2501.
2. Cremers, G. A.; et al. Efficient small-scale conjugation of DNA to primary antibodies for multiplexed cellular targeting. Bioconjugate chemistry. 2019, 30(9): 2384-2392.

For Research Use Only. NOT FOR CLINICAL USE.