How does the cleavable linker in the anti-MIgG(Fc)Fab-C-MMAF ADC enhance its functionality?

The cleavable linker in the anti-MIgG(Fc)Fab-C-MMAF ADC allows for the controlled release of MMAF upon internalization into target cells. This feature ensures that the cytotoxic agent is released directly within the cancer cells, enhancing the specificity and reducing systemic toxicity.

Can the anti-MIgG(Fc)Fab-C-MMAF ADC be used in preclinical settings?

Yes, the anti-MIgG(Fc)Fab-C-MMAF ADC is suitable for preclinical use, particularly for screening mouse monoclonal antibodies as potential ADC candidates. Its design allows for efficient and cost-effective preliminary assessments.

How does the Fc region of the anti-MIgG(Fc)Fab-C-MMAF ADC contribute to its efficacy?

The Fc region of the antibody enhances its binding affinity to Fc receptors on immune cells, facilitating antibody-dependent cellular cytotoxicity (ADCC) and other immune-mediated effects. This increases the therapeutic potential of the ADC.

What are the critical quality attributes of the anti-MIgG(Fc)Fab-C-MMAF ADC?

Critical quality attributes include the purity of the antibody fragment, the stability of the linker, and the activity of the MMAF. Ensuring these attributes are within specified limits is essential for maintaining the efficacy and safety of the ADC.

What types of cancer can the anti-MIgG(Fc)Fab-C-MMAF ADC target?

While the anti-MIgG(Fc)Fab-C-MMAF ADC is primarily used for screening and preclinical evaluations, it is designed to target cells that express specific antigens recognized by the primary antibody to which it is conjugated.

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anti-MIgG(Fc)Fab-C-MMAF ADC

anti-MIgG(Fc)Fab-C-MMAF ADC (CAT#: ADC-AA-028)

This ADC product is comprised of a Fab fragment of an anti-mouse IgG Fc specific polyclonal antibody conjugated via a cleavable linker to MMAF. The antibody portion is a Fab fragment of a secondary antibody and the drug portion, MMAF, is a cytotoxic small molecule which binds to tubulins, interrupts microtubule dynamics, and induces cell death. This product displays no obvious toxicity without a primary antibody and can be a quite efficient and economical alternative to pre-screening mouse monoclonal antibodies as ADC candidates.

ADC Target
ADC Antibody
ADC Linker
ADC payload drug

Name
IgG Fc

Overview
The fragment crystallizable region (Fc region) is composed of the constant region of the two heavy chains that form the IgG molecule. The Fc region of IgG bears a highly conserved N-glycosylation site. Glycosylation of the Fc fragment is essential for Fc receptor-mediated activity. Fc binds to various cell receptors and complement proteins thus mediating different physiological effects of antibodies, such as opsonization, antibody dependent cellular cytotoxicity (ADCC), degranulation of mast cells, basophils, eosinophils and other processes.

Overview
Fab fragment of anti-mouse IgG Fc specific polyclonal antibody

Species Reactivity
Mouse

Name
Cleavable linkers

Description
Cleavable linkers rely on the physiological stimuli, which mainly include chemically cleavable linkers and enzymatically cleavable linkers. Chemically cleavable linkers including acid-labile linkers and disulfide linkers. For acid-labile linkers, intracellular release of payloads relies on the different pH between endosomes/lysosomes and blood. The release of disulﬁde-linked drugs is controlled by the factors in intracellular environment. Enzymatically cleavable linkers, peptide linkers and β-glucuronide linkers, are sensitive to enzymes located in cytoplasm.

Name
MMAF (Monomethyl auristatin F)

Description
Derived from Auristatin,are water-soluble dolastatin analogs of dolastatin 10. Dolastatin 10 belongs to dolastatin family and it can powerfully bind to tubulin, thus inhibiting polymerization mediated through the binding to the vinca alkaloid binding domain, and causes cell to accumulate in metaphase arrest.

For Research Use Only. NOT FOR CLINICAL USE.

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Customer Reviews FAQ

Excellent

The anti-MIgG(Fc)Fab-C-MMAF ADC provided an effective means for screening our mouse monoclonal antibodies. It exhibited impressive specificity with no detectable toxicity in our primary assays.

Excellent

I've used several ADCs, but this one from Creative Biolabs stands out. It targets tubulins and kills cells efficiently, exactly what we need for our antibody screening.

Excellent

Highly recommend the anti-MIgG(Fc)Fab-C-MMAF for labs focusing on antibody development. It's a cost-effective tool for pre-screening candidates without the risk of toxicity.

Excellent

Utilizing this ADC has streamlined our research process, offering a robust method to assess and advance our antibody candidates effectively.

Excellent

Impressed by the minimal toxicity and high efficiency of the anti-MIgG(Fc)Fab-C-MMAF ADC. It's become a staple in our antibody testing protocols.

Q: 1: How does the cleavable linker in the anti-MIgG(Fc)Fab-C-MMAF ADC enhance its functionality?
A: The cleavable linker in the anti-MIgG(Fc)Fab-C-MMAF ADC allows for the controlled release of MMAF upon internalization into target cells. This feature ensures that the cytotoxic agent is released directly within the cancer cells, enhancing the specificity and reducing systemic toxicity.
Q: 2: Can the anti-MIgG(Fc)Fab-C-MMAF ADC be used in preclinical settings?
A: Yes, the anti-MIgG(Fc)Fab-C-MMAF ADC is suitable for preclinical use, particularly for screening mouse monoclonal antibodies as potential ADC candidates. Its design allows for efficient and cost-effective preliminary assessments.
Q: 3: How does the Fc region of the anti-MIgG(Fc)Fab-C-MMAF ADC contribute to its efficacy?
A: The Fc region of the antibody enhances its binding affinity to Fc receptors on immune cells, facilitating antibody-dependent cellular cytotoxicity (ADCC) and other immune-mediated effects. This increases the therapeutic potential of the ADC.
Q: 4: What are the critical quality attributes of the anti-MIgG(Fc)Fab-C-MMAF ADC?
A: Critical quality attributes include the purity of the antibody fragment, the stability of the linker, and the activity of the MMAF. Ensuring these attributes are within specified limits is essential for maintaining the efficacy and safety of the ADC.
Q: 5: What types of cancer can the anti-MIgG(Fc)Fab-C-MMAF ADC target?
A: While the anti-MIgG(Fc)Fab-C-MMAF ADC is primarily used for screening and preclinical evaluations, it is designed to target cells that express specific antigens recognized by the primary antibody to which it is conjugated.

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Same Target Same Linker Same Payload

CAT#	Product Name	Linker	Payload
ADC-AA-027	anti-MIgG(Fc)Fab-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-053	Protein A-MMAF ADC		MMAF (Monomethyl auristatin F)
ADC-AA-031	anti-MIgG(Fc)Fab-N-DM1 ADC	Noncleavable linkers	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)
ADC-AA-051	Protein G-Duocarmycin ADC		Duocarmycins
ADC-AA-021	anti-MIgG(Fc)-C-MMAE ADC	Cleavable linkers	MMAE (Monomethyl auristatin E)

CAT#	Product Name	Linker	Payload
ADC-AA-029	anti-MIgG(Fc)Fab-C-MMAE ADC	Cleavable linkers	MMAE (Monomethyl auristatin E)
ADC-AA-002	anti-HIgG(Fc)-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-062	Anti-HIgG(Fc)Fab-C-DX8951 ADC	Cleavable linkers	DX8953
ADC-AA-010	anti-HIgG(Fc)Fab-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-020	anti-MIgG(Fc)-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)

CAT#	Product Name	Linker	Payload
ADC-AA-002	anti-HIgG(Fc)-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-027	anti-MIgG(Fc)Fab-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-019	anti-MIgG(Fc)-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-009	anti-HIgG(Fc)Fab-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-W-491	Anti-TNFRSF17-Mc-MMAF ADC	Mc (maleimidocaproyl)	MMAF (Monomethyl auristatin F)

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