What is anti-MIgG(Fc)-N-MMAF ADC?

This ADC consists of an anti-mouse IgG Fc-specific polyclonal antibody conjugated through a noncleavable linker to monomethyl auristatin F (MMAF). MMAF is a cytotoxic agent that disrupts microtubule dynamics, leading to cell death.

What are the primary uses of anti-MIgG(Fc)-N-MMAF ADC in research?

This ADC is used primarily to target and kill cells that express mouse IgG Fc receptors, making it valuable in studying diseases where such expressions are a factor, such as in certain cancer and autoimmune disease models.

What are the potential applications of anti-MIgG(Fc)-N-MMAF ADC beyond cancer research?

Beyond oncology, this ADC could potentially be explored in the study of autoimmune diseases where selective depletion of certain immune cells could be beneficial. However, the applicability and safety in such contexts would require thorough investigation.

How does the anti-MIgG(Fc)-N-MMAF ADC affect cell cycle progression in experimental models?

The ADC delivers MMAF, a microtubule-disrupting agent, directly to target cells, causing arrest at the G2/M phase of the cell cycle. Researchers can use flow cytometry to monitor these effects, providing insights into how MMAF impacts cell division and apoptosis in specific cell populations.

What experimental controls are recommended when using anti-MIgG(Fc)-N-MMAF ADC in cell-based assays?

Researchers should include controls that lack the Fc receptor expression to ascertain the specificity of the ADC for Fc receptor-positive cells. Additionally, using an isotype control ADC that does not contain MMAF can help distinguish effects due to MMAF from those mediated solely by antibody binding.

Home
Products
Anti-Ab ADCs
IgG Fc
anti-MIgG(Fc)-N-MMAF ADC

anti-MIgG(Fc)-N-MMAF ADC (CAT#: ADC-AA-019)

This ADC product is comprised of an anti-mouse IgG Fc specific polyclonal antibody conjugated via a noncleavable linker to MMAF. The antibody portion is a secondary antibody and the drug portion, MMAF, is a cytotoxic small molecule which binds to tubulins, interrupts microtubule dynamics, and induces cell death. This product displays no obvious toxicity without a primary antibody and can be a quite efficient and economical alternative to pre-screening mouse monoclonal antibodies as ADC candidates.

ADC Target
ADC Antibody
ADC Linker
ADC payload drug

Name
IgG Fc

Overview
The fragment crystallizable region (Fc region) is composed of the constant region of the two heavy chains that form the IgG molecule. The Fc region of IgG bears a highly conserved N-glycosylation site. Glycosylation of the Fc fragment is essential for Fc receptor-mediated activity. Fc binds to various cell receptors and complement proteins thus mediating different physiological effects of antibodies, such as opsonization, antibody dependent cellular cytotoxicity (ADCC), degranulation of mast cells, basophils, eosinophils and other processes.

Overview
anti-mouse IgG Fc specific polyclonal IgG antibody

Species Reactivity
Mouse

Name
Noncleavable linkers

Description
Noncleavable linkers, is considered noncleavable-meaning linker cleavage, and payload release does not depend on the differential properties between the plasma and some cytoplasmic compartments. Instead, the release of the cytotoxic drug is postulated to occur after internalization of the ADC via antigen-mediated endocytosis and delivery to lysosomal compartment, where the antibody is degraded to the level of amino acids through intracellular proteolytic degradation.

Name
MMAF (Monomethyl auristatin F)

Description
Derived from Auristatin,are water-soluble dolastatin analogs of dolastatin 10. Dolastatin 10 belongs to dolastatin family and it can powerfully bind to tubulin, thus inhibiting polymerization mediated through the binding to the vinca alkaloid binding domain, and causes cell to accumulate in metaphase arrest.

For Research Use Only. NOT FOR CLINICAL USE.

Published Data

+ Submit Publications

Submit a review or a question

Scientific Resources

CAMouse™-Fully Human Antibody Generation Platform

Reviews

Literature

Protocol

Customer Reviews and FAQs

There are currently no Customer reviews or questions for ADC-AA-019. Click the button above to contact us or submit your feedback about this product.

Customer Reviews FAQ

Excellent

Anti-MIgG(Fc)-N-MMAF ADC has been a game-changer in our lab. Excellent specificity and potency with no toxicity, making it ideal for mouse antibody screening.

Excellent

Highly recommend this ADC

Anti-MIgG(Fc)-N-MMAF provides precision targeting with MMAF, disrupting tubulin effectively and safely in our cellular models.

Excellent

Creative Biolabs' ADC offers outstanding results with its targeted approach using MMAF. It's significantly improved our efficiency in monoclonal antibody development.

Excellent

Anti-MIgG(Fc)-N-MMAF ADC is a robust tool in our research arsenal. Its precise mechanism and cytotoxic action are invaluable for studying antibody-mediated effects.

Excellent

This product's ability to bind specifically and induce cell death without off-target effects makes it a top choice for our monoclonal antibody screenings.

Q: 1: What is anti-MIgG(Fc)-N-MMAF ADC?
A: This ADC consists of an anti-mouse IgG Fc-specific polyclonal antibody conjugated through a noncleavable linker to monomethyl auristatin F (MMAF). MMAF is a cytotoxic agent that disrupts microtubule dynamics, leading to cell death.
Q: 2: What are the primary uses of anti-MIgG(Fc)-N-MMAF ADC in research?
A: This ADC is used primarily to target and kill cells that express mouse IgG Fc receptors, making it valuable in studying diseases where such expressions are a factor, such as in certain cancer and autoimmune disease models.
Q: 3: What are the potential applications of anti-MIgG(Fc)-N-MMAF ADC beyond cancer research?
A: Beyond oncology, this ADC could potentially be explored in the study of autoimmune diseases where selective depletion of certain immune cells could be beneficial. However, the applicability and safety in such contexts would require thorough investigation.
Q: 4: How does the anti-MIgG(Fc)-N-MMAF ADC affect cell cycle progression in experimental models?
A: The ADC delivers MMAF, a microtubule-disrupting agent, directly to target cells, causing arrest at the G2/M phase of the cell cycle. Researchers can use flow cytometry to monitor these effects, providing insights into how MMAF impacts cell division and apoptosis in specific cell populations.
Q: 5: What experimental controls are recommended when using anti-MIgG(Fc)-N-MMAF ADC in cell-based assays?
A: Researchers should include controls that lack the Fc receptor expression to ascertain the specificity of the ADC for Fc receptor-positive cells. Additionally, using an isotype control ADC that does not contain MMAF can help distinguish effects due to MMAF from those mediated solely by antibody binding.

Quick Links

ADC Toxins

Drug-linker Complexes

Customized ADCs

Anti-Ab ADCs

Other Products

Same Target Same Linker Same Payload

CAT#	Product Name	Linker	Payload
ADC-AA-049	Protein G-MMAF ADC		MMAF (Monomethyl auristatin F)
ADC-AA-050	Protein G-MCC-DM1 ADC	MCC (Maleimidomethyl cyclohexane-1-carboxylate)	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)
ADC-AA-053	Protein A-MMAF ADC		MMAF (Monomethyl auristatin F)
ADC-AA-055	Protein A-Duocarmycin ADC		Duocarmycins
ADC-AA-029	anti-MIgG(Fc)Fab-C-MMAE ADC	Cleavable linkers	MMAE (Monomethyl auristatin E)

CAT#	Product Name	Linker	Payload
ADC-AA-039	anti-Rat IgG(Fc)-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-031	anti-MIgG(Fc)Fab-N-DM1 ADC	Noncleavable linkers	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)
ADC-AA-063	Anti-HIgG(Fab)-N-MMAF ADC	Noncleavable linkers	MMAF
ADC-AA-045	anti-Rat IgG(HL)-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-037	anti-RIgG(HL)Fab-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)

CAT#	Product Name	Linker	Payload
ADC-W-515	Anti-EGFR-Mc-MMAF ADC-5	Mc (maleimidocaproyl)	MMAF (Monomethyl auristatin F)
ADC-W-493	Anti-CD19-Mc-MMAF ADC-3	Mc (maleimidocaproyl)	MMAF (Monomethyl auristatin F)
ADC-AA-002	anti-HIgG(Fc)-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-W-604	Anti-FUT3-Mc-VC-PABC-MMAF ADC-3	MC-VC-PABC (maleimidocaproyl-valine-citrulline-p-aminobenzoyloxycarbonyl)	MMAF (Monomethyl auristatin F)
ADC-AA-049	Protein G-MMAF ADC		MMAF (Monomethyl auristatin F)

Online Inquiry

Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.