Agonistic Antibody Screening Service

Biologics, especially therapeutic monoclonal antibodies, have been very successful in the last decade or so. Among them, agonistic monoclonal antibodies against certain targets can effectively enhance the immune response and become one of the most effective therapies against cancer. Creative Biolabs' antibody development team is dedicated to screening and identifying novel agonistic antibody for our clients.

Creative Biolabs uses a highly diverse phage display library to speed up your agonist antibody screening program, giving you access to purified recombinant antibodies in less than 2 months. Our large-capacity human phage library is composed of a variety of monoclonal antibody formats, including Fab, scFv or VHH and multiple species, which allows us to develop therapeutic antibodies without the need for humanization.

Background of Antibody Phage Display Technology

Phage display is a powerful and widely used laboratory technique for studying protein-protein, protein-peptide and protein-DNA interactions. This technique is based on the fact that the gene encoding the displayed molecule is packaged in the same virus as a single strained DNA (ssDNA) and the displayed peptide or protein is expressed in fusion with the phage coat protein.

Our phage display technology is based primarily on libraries of millions or even billions of phage particles, screening for conjugates with high affinity and specificity. It allows the creation of libraries containing up to 10¹² different variants that can be used to affinity screen combinatorial peptide libraries to study protein-ligand interactions and to characterize these ligands.

Fig.1 Phage display cycle.

Phage Display Based Agonistic Antibody Screening

Based on an advanced phage display technology platform, Creative Biolabs can produce high affinities and high specificity agonist formats, such as sdAbs, diabodies, chimeric antibodies (such as humanized Antibodies), VHH single domain antibodies. Three phage display systems are available, including M13 phage display system, T4 phage display system and T7 phage display system. Our scientists typically generate a library of 10⁸ -10⁹ levels of diversity, or sometimes more than 10¹⁰ libraries or custom library capacity to meet specific requirements.

	M13	T4	T7
Genome Size	6407 bp	168895 bp	39937 bp
Display Protein	pVI, pIII and pVIII	SOC and HOC	gp10B
Display Size	>110 kDa on pIII	<710 kDa	<132 kDa
Display Size	<10 kDa on pVIII	<710 kDa	<132 kDa
Display Density	<5 copies on pIII	<810 copies on SOC	<415 copies
Display Density	<2700 copies on pVIII	<155 copies on HOC	<415 copies
Lifecycle	Lysogeny	Lytic	Lytic

Table 1. Our phage display library and their characteristics.

Advantages of Antibody Phage Display

Simplified structure
Stability
Reliability of phages
Applied to various surfaces
Applied to in vivo applications.

Creative Biolabs has been working in the field of antibody engineering for more than a decade, and our scientists are fully confident of completing the most challenging antibody engineering projects. For additional details of antibody screening services, please contact us.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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