Follow us on:
Dedication / Services
To provide 50000 services and products to global scientific research units every year

amplification of Fab expression cassette...

  1. cDNA is quantified by UV spectrometry as below.
  2. The rabbit VH and VK/VL genes are amplified from cDNA pool by the first round of PCR
  3. Both human CK and human CH1 genes are amplified from pDisplay-2 by the 2nd round of PCR.
  4. Rabbit VK/VL genes are assembled with human CK by conventional overlapping PCR. The product is named as light chain cassette.
  5. Rabbit VH genes are assembled with human CH1 by conventional overlapping PCR. The product is named as heavy chain cassette.
  6. The light chain cassette and the heavy chain cassette are assembled into Fab casstte by over-lapping PCR too.

The primers and the schematic process above are shown in appendices section.

Our customer service representatives are available 24 hours a day, from Monday to Sunday. Contact Us

Online Inquiry
*E-mail Address:
*Service & Products Interested:
Project Description:
*Verification Code:
Please input "biolabs"(case insensitive) as verification code.
Contact Us

Tel: 1-631-381-2994
Fax: 1-631-207-8356

Tel: 44-207-097-1828


Phage Display Platform Membrane Protein Platform Hyperdoma™ Platform Yeast Display Platform
© 2007 - 2018 Creative-Biolabs All Rights Reserved