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ANO2 Membrane Protein Introduction

Introduction of ANO2

ANO2 (Anoctamin-2), also known as Transmembrane member 16B (TMEM16B), is a protein that, in humans, is encoded by the ANO2 gene. It functions as Ca2+-activated Cl channels (CaCCs), a type of ion channel that plays important functions such as transepithelial ion transport, smooth muscle contraction, olfaction, phototransduction, nociception, and control of neuronal excitability.

Basic Information of ANO2
Protein Name Anoctamin-2
Gene Name ANO2
Aliases C12orf3, TMEM16B
Organism Homo sapiens (Human)
UniProt ID Q9NQ90
Transmembrane Times 8
Length (aa) 1003
Sequence MATPGPRDIPLLPGSPRRLSPQAGSRGGQGPKHGQQCLKMPGPRAPGLQGGSNRDPGQPCGGESTRSSSVINNYLDANEPVSLEARLSRMHFHDSQRKVDYVLAYHYRKRGVHLAQGFPGHSLAIVSNGETGKEPHAGGPGDIELGPLDALEEERKEQREEFEHNLMEAGLELEKDLENKSQGSIFVRIHAPWQVLAREAEFLKIKVPTKKEMYEIKAGGSIAKKFSAALQKLSSHLQPRVPEHSNNKMKNLSYPFSREKMYLYNIQEKDTFFDNATRSRIVHEILKRTACSRANNTMGINSLIANNIYEAAYPLHDGEYDSPEDDMNDRKLLYQEWARYGVFYKFQPIDLIRKYFGEKIGLYFAWLGLYTSFLIPSSVIGVIVFLYGCATIEEDIPSREMCDQQNAFTMCPLCDKSCDYWNLSSACGTAQASHLFDNPATVFFSIFMALWATMFLENWKRLQMRLGYFWDLTGIEEEEERAQEHSRPEYETKVREKMLKESNQSAVQKLETNTTECGDEDDEDKLTWKDRFPGYLMNFASILFMIALTFSIVFGVIVYRITTAAALSLNKATRSNVRVTVTATAVIINLVVILILDEIYGAVAKWLTKIEVPKTEQTFEERLILKAFLLKFVNAYSPIFYVAFFKGRFVGRPGSYVYVFDGYRMEECAPGGCLMELCIQLSIIMLGKQLIQNNIFEIGVPKLKKLFRKLKDETEAGETDSAHSKHPEQWDLDYSLEPYTGLTPEYMEMIIQFGFVTLFVASFPLAPVFALLNNVIEVRLDAKKFVTELRRPDAVRTKDIGIWFDILSGIGKFSVISNAFVIAITSDFIPRLVYQYSYSHNGTLHGFVNHTLSFFNVSQLKEGTQPENSQFDQEVQFCRFKDYREPPWAPNPYEFSKQYWFILSARLAFVIIFQNLVMFLSVLVDWMIPDIPTDISDQIKKEKSLLVDFFLKEEHEKLKLMDEPALRSPGGGDRSRSRAASSAPSGQSQLGSMMSSGSQHTNV

Function of ANO2 Membrane Protein

As a calcium-activated chloride channel, ANO2 may play a role in olfactory signal transduction. Odorant molecules bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells. ANO2 seems to be the underlying chloride channel involved in this process. In addition, ANO2 may mediate light perception amplification in the retina. It’s also identified as an autoimmune target found in multiple sclerosis. Furthermore, ANO2 has been identified as an autoimmune target in multiple sclerosis (MS) and the pathogenic role of ANO2 autoantibodies in MS has been revealed.

TMEM16A and TMEM16B function in neurons.Fig.1 TMEM16A and TMEM16B function in neurons. (Pedemonte, 2014)

Application of ANO2 Membrane Protein in Literature

  1. Whitlock J.M and Hartzell H.C. Anoctamins/TMEM16 Proteins: Chloride Channels Flirting with Lipids and Extracellular Vesicles. Annu Rev Physiol. 2017, 79:119-143. PubMed ID: 27860832

    This article discusses the physiological implications of Ca2+-dependent phospholipid scrambling, the extracellular vesicles associated with this activity, and the roles of ANOs in these processes.

  2. Ayoglu B., et al. Anoctamin 2 identified as an autoimmune target in multiple sclerosis. Proc Natl Acad Sci U S A. 2016, 113(8):2188-93. PubMed ID: 26862169

    This article demonstrates that an ANO2 autoimmune subphenotype may exist in MS and lay the groundwork for further studies focusing on the pathogenic role of ANO2 autoantibodies in MS.

  3. Pedemonte N and Galietta L.J. Structure and function of TMEM16 proteins (anoctamins). Physiol Rev. 2014, 94(2):419-59. PubMed ID: 24692353

    This study suggests that dual function is an ancestral characteristic of TMEM16 proteins and that some members, such as TMEM16A and TMEM16B/ANO2, have evolved to a pure channel function.

  4. Stephan A.B., et al. ANO2 is the cilial calcium-activated chloride channel that may mediate olfactory amplification. Proc Natl Acad Sci U S A. 2009, 106(28):11776-81. PubMed ID: 19561302

    This article proposes that ANO2 constitutes the olfactory calcium-activated chloride channel.

  5. Tian Y., et al. Anoctamins are a family of Ca2+-activated Cl- channels. J Cell Sci. 2012, 125(Pt 21):4991-8. PubMed ID: 22946059

    This article demonstrates that anoctamins are a family of Ca(2+)-activated Cl(-) channels, which also induce permeability for cations. They may operate as Cl(-) channels located in the plasma membrane or in intracellular compartments.

ANO2 Preparation Options

We provide custom membrane protein preparation services for worldwide customers. Leveraging by our advanced Magic™ membrane protein production platform, we are able to present target membrane protein in multiple active formats. Our professional scientists are happy to help you find an ideal method and make your project a success. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-ANO2 antibody development services.


Creative Biolabs provides high-quality membrane protein preparation service to facilitate the development of worldwide customer’s research. During the past years, we have successfully established a powerful Magic™ membrane protein platform which enables us to provide a series of membrane protein preparation services. For more detailed information, please feel free to contact us.

Reference

  1. Pedemonte N and Galietta L J. (2014). Structure and function of TMEM16 proteins (anoctamins). Physiol Rev. 94(2):419-59.

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