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ANO7 Membrane Protein Introduction

Introduction of ANO7

ANO7 is encoded by the ANO7 gene. It is also known as TMEM16G and is a member of transmembrane protein 16 (TMEM16) family, which is responsible for a variety of physiological functions, such as ion transport, phospholipid scrambling and regulating other ion channels. The crystal structure of ANO7 has not been solved so far. The proposed structural model according to the amino acid sequence indicates that ANO7 possesses eight predicted transmembrane helices. Meanwhile, ANO7 is also known as Dresden-transmembrane protein of the prostate (D-TMPP).

Basic Information of ANO7
Protein Name Anoctamin-7
Gene Name ANO7
Aliases Dresden transmembrane protein of the prostate, D-TMPP, IPCA-5, Prostate cancer-associated protein 5, Transmembrane protein 16G, TMEM16G
Organism Homo sapiens (Human)
UniProt ID Q6IWH7
Transmembrane Times 8
Length (aa) 933
Sequence MRMAATAWAGLQGPPLPTLCPAVRTGLYCRDQAHAERWAMTSETSSGSHCARSRMLRRRAQEEDSTVLIDVSPPEAEKRGSYGSTAHASEPGGQQAAACRAGSPAKPRIADFVLVWEEDLKLDRQQDSAARDRTDMHRTWRETFLDNLRAAGLCVDQQDVQDGNTTVHYALLSASWAVLCYYAEDLRLKLPLQELPNQASNWSAGLLAWLGIPNVLLEVVPDVPPEYYSCRFRVNKLPRFLGSDNQDTFFTSTKRHQILFEILAKTPYGHEKKNLLGIHQLLAEGVLSAAFPLHDGPFKTPPEGPQAPRLNQRQVLFQHWARWGKWNKYQPLDHVRRYFGEKVALYFAWLGFYTGWLLPAAVVGTLVFLVGCFLVFSDIPTQELCGSKDSFEMCPLCLDCPFWLLSSACALAQAGRLFDHGGTVFFSLFMALWAVLLLEYWKRKSATLAYRWDCSDYEDTEERPRPQFAASAPMTAPNPITGEDEPYFPERSRARRMLAGSVVIVVMVAVVVMCLVSIILYRAIMAIVVSRSGNTLLAAWASRIASLTGSVVNLVFILILSKIYVSLAHVLTRWEMHRTQTKFEDAFTLKVFIFQFVNFYSSPVYIAFFKGRFVGYPGNYHTLFGVRNEECAAGGCLIELAQELLVIMVGKQVINNMQEVLIPKLKGWWQKFRLRSKKRKAGASAGASQGPWEDDYELVPCEGLFDEYLEMVLQFGFVTIFVAACPLAPLFALLNNWVEIRLDARKFVCEYRRPVAERAQDIGIWFHILAGLTHLAVISNAFLLAFSSDFLPRAYYRWTRAHDLRGFLNFTLARAPSSFAAAHNRTCRYRAFRDDDGHYSQTYWNLLAIRLAFVIVFEHVVFSVGRLLDLLVPDIPESVEIKVKREYYLAKQALAENEVLFGTNGTKDEQPEGSELSSHWTPFTVPKASQLQQ

Function of ANO7 Membrane Protein

ANO7 is expressed in many different cell types, including epithelia and neurons. ANO7 functions as ion channels like the majority of TMEM16 proteins. It is involved in epithelial Cl- transport, smooth muscle contraction and neuronal signal processing. ANO7 is reported to conduct Cl- influx or Cl- efflux, and the balance is regulated by the dynamic system of intracellular chloride, which is decided by the various Cl-/cation co-transporters and their regulatory proteins. Moreover, ANO7 can work as lipid scramblases and catalyze the diffusion of lipids between the two leaflets of a bilayer. Even though the activity of TMEM16 has been implemented in a number of diseases including hypertension, asthma, diarrhoea, cancer and more recently ischaemia-induced arrhythmia, there is no non-selective nature of the pharmacology surrounding TMEM16 has impeded our knowledge of these proteins.

Proposed structure of ANO7 membrane protein Fig.1 Proposed structure of ANO7 membrane protein (Brunner, 2014).

Application of ANO7 Membrane Protein in Literature

  1. Brunner J.D., et al. X-ray structure of a calcium-activated TMEM16 lipid scramblase. Nature. 2014, 516:207. PubMed ID: 25383531

    This article reveals the general architecture of the TMEM16 family and its mode of Ca2+ activation. It serves as a framework to unravel the conduction of ions in certain TMEM16 proteins.

  2. Pedemonte N., et al. Structure and Function of TMEM16 Proteins (Anoctamins). Physiol. Rev. 2014, 94(2):419-459. PubMed ID: 24692353

    The article reports that some mutations in anoctamin genes (ANO3, ANO5, ANO6, ANO7 and ANO10) result in various genetic diseases and suggests the involvement of anoctamins in a variety of cell functions whose link with ion transport and/or lipid scrambling.

  3. Katoh, M., et al. Characterization of human TMEM16G gene in silico. International Journal of Molecular Medicine. 2004, 759-823. PubMed ID: 15375614

    The authors in this article apply the bioinformatic method to characterize the human ANO7 gene, which is consisted of 25 exons and located at human chromosome 2q37.3.

  4. Kiessling A., et al. D-TMPP: A novel androgen-regulated gene preferentially expressed in prostate and prostate cancer that is the first characterized member of an eukaryotic gene family. The Prostate. 2005, 64(4):387-400. PubMed ID: 15761874

    This article reveals that the mRNA of ANO7 encodes a putative multiple transmembrane protein of 883 amino acids and is selectively overexpressed in prostate tissue.

  5. Picollo A., et al. TMEM16 proteins: unknown structure and confusing functions. J. Mol. Biol. 2015, 427(1):94-105. PubMed ID: 25451786

    This article reviews the unknown structure and confusing functions of the TMEM16 family of membrane proteins and the links between TMEM16 functions and human physiology and pathologies need to be investigated.

ANO7 Preparation Options

To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-ANO7 antibody development services.


Creative Biolabs, a forward-looking research institute as well as a leading customer service provider in the field of membrane protein. We are famous for offering comprehensive, integrated and highly customizable services for successfully accomplishing numerous challenging projects including generation of many functional membrane proteins. Please feel free to contact us for more information.

Reference

  1. Brunner JD., et al. (2014) X-ray structure of a calcium-activated TMEM16 lipid scramblase. Nature 516:207.

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