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HighlightsIntroduction of ANO8
ANO8 is also known as Anoctamin-8, transmembrane protein 16H and TMEM16H. It belongs to the transmembrane protein 16 (TMEM16) family which is a large family of membrane proteins and consists of ten members (TMEM16A, B, C, D, E, F, G, H, J and K) in mammals. The TMEM16 family of proteins has been identified as Ca2+ activated Cl− channels and all homologues might share a similar function. Meanwhile, the predicted structure of ANO8 indicates that ANO8 have eight transmembrane domains.
| Basic Information of ANO8 | |
| Protein Name | Anoctamin-8 |
| Gene Name | ANO8 |
| Aliases | Transmembrane protein 16H, TMEM16H |
| Organism | Homo sapiens (Human) |
| UniProt ID | Q9HCE9 |
| Transmembrane Times | 8 |
| Length (aa) | 1232 |
| Sequence | MAEAASGAGGTSLEGERGKRPPPEGEPAAPASGVLDKLFGKRLLQAGRYLVSHKAWMKTVPTENCDVLMTFPDTTDDHTLLWLLNHIRVGIPELIVQVRHHRHTRAYAFFVTATYESLLRGADELGLRKAVKAEFGGGTRGFSCEEDFIYENVESELRFFTSQERQSIIRFWLQNLRAKQGEALHNVRFLEDQPIIPELAARGIIQQVFPVHEQRILNRLMKSWVQAVCENQPLDDICDYFGVKIAMYFAWLGFYTSAMVYPAVFGSVLYTFTEADQTSRDVSCVVFALFNVIWSTLFLEEWKRRGAELAYKWGTLDSPGEAVEEPRPQFRGVRRISPITRAEEFYYPPWKRLLFQLLVSLPLCLACLVCVFLLMLGCFQLQELVLSVKGLPRLARFLPKVMLALLVSVSAEGYKKLAIWLNDMENYRLESAYEKHLIIKVVLFQFVNSYLSLFYIGFYLKDMERLKEMLATLLITRQFLNVREVLQQPHLYRRLGRGELGLRAVWELARALLGLLSLRRPAPRRLEPQADEGGGGGSGGGGRRCLSGGCGAPEEEEEAALVERRRAGEGGEEGDGPPGGKEEDEDDEEEEDEEEEEDEEEGEEGGLLDCGLRLKKVSFAERGAGRRRPGPSPEALLEEGSPTMVEKGLEPGVFTLAEEDDEAEGAPGSPEREPPAILFRRAGGEGRDQGPDGGPDPEPGSNSDSTRRQRRQNRSSWIDPPEEEHSPQLTQAELESCMKKYEDTFQDYQEMFVQFGYVVLFSSAFPLAALCALVNNLIEIRSDAFKLCTGLQRPFGQRVESIGQWQKVMEAMGVLAIVVNCYLIGQCGQLQRLFPWLSPEAAIVSVVVLEHFALLLKYLIHVAIPDIPGWVAEEMAKLEYQRREAFKRHERQAQHRYQQQQRRRREEEERQRHAEHHARREHDSGGREEARAEGSGLDPATSSEKASAKAKGSTAGGHGPERPKRPGSLLAPNNVMKLKQIIPLQGKFLSSGATSSLAAAGAGATTRPPPAQSPTGSDTRLPAFLSFKFLKSPETRRDSERSHSPPKAFHAGKLFPFGGTRAEPGSNGAGGQARPDGTPSSGSSRVQRSGPVDEALAEELEAPRPEEEGSGTALAPVGAPALRTRRSRSPAPPPPMPLPRPPTPPAGCWQWDGPWGCGGEGAAPRQALAAAECPPCAMAGPPPAPQPLPGDASFYSLPPPPLPPTSDPLETPAPSPSPSPSPQAVCWPSGWH |
Function of ANO8 Membrane Protein
ANO8 belongs to the TMEM16 family, which functions as Ca2+-activated Cl− channels. ANO8 mRNA is expressed in all electrolyte transporting tissues like trachea, pancreas, colon, salivary gland, prostate tissue and the thyroid gland. Compared with ANO1, expression of the other epithelial anoctamins ANO8 can be induced by very little Ca2+ (ATP)-activated Cl- conductance. In cells expressing ANO8, whole-cell currents of variable magnitudes are activated by ATP and the activation of ANO8 by ATP can induce the increase of the intracellular Ca2+. The increase of Ca2+ may not only activate the Cl- channel, but also trigger inhibition of anoctamins via CAMKII, which may actually counteract further activation. CAMKII is a multi-functional kinase that controls a range of cellular functions, including proliferation, differentiation and apoptosis. The inhibition of anoctamins via CAMKII suggests that ANO8 is involved in the signaling pathway of CAMKII. However, the detailed mechanism remains to be solved.
Fig.1 Possible structure of ANO8 membrane protein (Andra, 2018).
Application of ANO8 Membrane Protein in Literature
This paper reports the identification and characterization of the ANO8 gene as well as on the molecular evolution of ANO8 for the first time.
The article reviews that the newly described anoctamin family and some members of this family are up-regulated in a number of tumors and functional deficiency in others is linked to developmental defects.
This article focuses on the anoctamin protein paralogs evolved from several gene duplication events followed by functional divergence of vertebrate anoctamins. The results of this article suggest that anoctamins have evolved by a series of duplication events, and that they are constrained by purifying selection.
The results of this article demonstrate that anoctamins are a family of Ca2+ activated Cl− channels and increase our understanding of the physiological significance of anoctamins and their role in disease.
The authors of this article analyze the expression pattern of all ten members (ANO1-ANO10) in a broad range of murine tissues and detected predominant expression of ANO1, 6, 7, 8, 9, 10 in epithelial tissues, while ANO2, 3, 4, 5 are common in neuronal and muscle tissues.
ANO8 Preparation Options
To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-ANO8 antibody development services.
Creative Biolabs is founded by scientists who are dedicated to providing comprehensive, integrated and highly customizable services in the field of membrane protein. We are famous for successfully accomplishing numerous challenging projects including generation of many functional membrane proteins. Please feel free to contact us for more information.
Reference
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