Having extensive experience in the field of antibody research, Creative Biolabs is proud to offer our clients a series of anti-carbohydrate antibody development services with the best quality and most competitive price. Aided by our state-of-the-art technology and professional scientists, we are able to help global customers produce anti-carbohydrate antibody, including monoclonal antibody and polyclonal antibody.
Natural antibodies (NAb) are defined as those immunoglobulins (mainly IgM isotype) continuously secreted by B1 type lymphocytes without any previous external antigen stimulation. To date, a broad range of NAb target carbohydrates (NAbC) have been characterized and can be divided into three different groups: 1) conservative NAbC that are practically the same regarding specificity and blood levels in all healthy individuals; 2) alloantibodies to blood group antigens and xenoantibodies to antigens from other species; 3) plastic antibodies, which include antibodies from the two previous groups but with variable levels during certain conditions, such as pregnancy or inflammation. In addition, studies have shown that some NAbCs could react to tumor-associated carbohydrate antigens, which are associated with worse tumor progression.
Fig.1 Plasma-derived anti-glycan antibodies of IgG and IgM isotype bind to polymeric presented Globo H glyco-clusters differentiating healthy controls from ovarian cancer comparable to CA125. (Pochechueva T, 2017)
Recently, many anti-carbohydrate antibodies have been applied in the diagnostic and treatment of tumors. Therefore, there is an urgency to produce more and more antibodies to target various carbohydrate antigens. However, immunization with carbohydrates often leads to a primary IgM response and even no response in some cases as many carbohydrates are self-antigens. In this case, traditional hybridoma technology may have a limitation in the production of anti-carbohydrate antibodies. Based on our advanced hybridoma platform, Creative Biolabs has developed an efficient method of creating anti-carbohydrate monoclonal antibodies by immunizing mice that lack the enzymes to synthesize target glycans. What’s more, our phage display provides another strategy to produce antibodies directed against various carbohydrate moieties since conventional hybridoma technologies have yielded mostly low-affinity antibodies against a limited number of carbohydrate antigens.
Equipped with our top antibody production platform, Creative Biolabs is able to offer a full range of anti-carbohydrate antibody production services, which including but not limited to:
With extensive experience in antibody generation, Creative Biolabs is confident in offering the best services in a timely and cost-effective manner. Meanwhile, we also provide other services regarding anti-carbohydrate antibody development. For more information, please feel free to contact us.
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