Anti-Membrane Protein Antibody Discovery Using Camel Phage Display Immune Library

With years of experience and professional scientific team, Creative Biolabs proudly introduces our world-leading anti-membrane protein antibody discovery service using phage display immune library in camel.

Membrane proteins, such as GPCRs and ion channels, have been gaining increasing research attention in recent years. They represent one major drug targets of more than 50% modern drugs due to their irreplaceable roles in physiological activities. Dysfunction of key membrane proteins can be highly relevant to almost diverse diseases, such as cancers and infections. In contrast to small molecular drugs, monoclonal antibodies exhibit many unique advantages, e.g. higher affinity, higher specificity, longer lasting time in blood and lower cytotoxicity, etc. However, to develop mAb drugs can be very challenging, especially against membrane proteins. As is known, membrane proteins are hard to obtain due to their complicated structures, large transmembrane hydrophobic domains, and complex function profiles. On the other hand, how to generate good binders with desired functional profile is also of great significance. In order to address these challenges, Creative Biolabs has built a versatile Magic™ anti-membrane protein antibody discovery platforms, based on which we have successfully delivered many superior mAbs against membrane targets in a robust and reliable way.

Antibodies derived from camelids are a unique type of antibodies that consist of heavy chains only (without light chains). The N terminal variable region of the heavy chain (V_HH) is capable of antigen binding. Compared to antigen-binding entities of classical antibodies, camel V_HH offers a number of benefits in medical and biotechnological applications including but not limited to:

Smaller size

The MW of V_HH is only 12~15 kDa while traditional IgG is 146 kDa. Therefore, V_HH antibody can across blood-brain barriers (BBB) and have easy access to many conformational epitopes. In addition, small-size V_HHs are also easier to synthesize in economic prokaryotic hosts, while Fabs/IgGs often requires expensive mammalian exȐion.

Higher thermal and chemical stability

V_HH displays better resistance against extreme pH/temperature. Some V_HH are able to bind corresponding antigen at 90°C, which is not possible for conventional antibodies.

Strictly monomeric behavior

In contrast to scFv that often dimerizes to scFv₂, V_HH shows no sign of spontaneous dimerization. Hence they are more homogeneous.

Incorporated with powerful phage display technique, Creative Biolabs now offers the first-class service of camel antibody development against membrane protein targets. Our one-stop service package can start from antigen design and preparation. Aided by our well-established membrane protein immunogen preparation platform, we offer flexible options (liposomes, polymers, nanodiscs, designed peptides, DNA immunization, whole cell presentation, etc.), from which we can find the best fit for every specific target. We also present versatile screening strategies, e.g. solution-sorting screening, solid phase screening, cell-based screening. Every factor and requirement from clients will be taken into consideration to ensure the optimal outcome.

Fig 1 Generation of sdAb using phage display from camel libraries. (Zafra O. et al. 2011)

Creative Biolabs is specialized in tailoring superior camel anti-membrane protein antibodies using phage display approach. With numerous successful cases in anti-ion channel antibody production, anti-GPCR antibody production, and anti-transporter antibody production, we are more than glad to leverage our knowledge and technologies to address custom demands. Please feel free to contact us for more details, and we will be here for you 24/7.

Reference

Zafra O, Fraile S, Gutiérrez C, et al. (2011). Monitoring biodegradative enzymes with sdAbs raised in Camelus dromedarius with mixtures of catabolic proteins. Environmental Microbiology. 13(4):960-974.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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