As one of the world's leading providers of antibody discovery services, Creative Biolabs has been constantly dedicated to assisting our customers with their diverse development projects. Particularly, we offer professional anti-membrane protein antibody discovery services using mouse phage display immune library method.
Membrane proteins, such as GPCRs and ion channels, participate in diverse key cellular functions. Many diseases, e.g. cancers, autoimmune diseases, infection diseases, accompanied by the dysfunction of membrane proteins. Discovery of anti-membrane protein antibody takes a crucial part in diverse therapeutic and diagnostics research. Hybridoma technology represents a classic and well-developed approach, but the limitations in fusion efficacy and host species often pose as a big challenge. In contrast, phage display technology provides a promising alternative to generate high-specific, high-affinity antibody binders.
The unique characteristic of phenotype-genotype linkage makes phage display a robust and straightforward strategy for antibody discovery. Antibody phage display libraries derived from immunized mice is proved as an attractive approach in this regard. Creative Biolabs provides the world first-class phage display library construction services for years. Based on this platform, a large number of promising clones have been identified, some of which have already entered clinical stages. Besides, the small size, high reproduction rate of mice and easiness of maintenance and handling make them the top popular mammalian animal models in laboratory.
Advantages of anti-membrane protein antibodies discovery using mouse immune library:
Mice and humans share about 97.5% of their working DNA. According to whole genome sequencing analysis, it is revealed that there are only 21 human genes that do not have a direct counterpart in mouse genome, and only 14 genes are unique to mice that are not found in humans.
We have integrated multiple transgenic mouse strains, which can be used for different research purpose. For example, on our Speed™ anti-membrane protein antibody development platform, we developed unique gene-modified mice which can raise significant immune responses in only 20-day period. We also have a Magic™ humanized transgenic mice platform, on which 100% human antibodies can be generated.
Creative Biolabs has well-established membrane protein immunogen preparation platform, specifically designed for membrane antigens. A variety of expression systems, stabilization format, modifications can be applied to tailor most appreciate immunogen. In addition, we also provide a highly effective adjuvant to ensure strong immunity in vivo.
Creative Biolabs has been long dedicated to anti-membrane protein antibody development using various approaches (e.g. hybridoma, B cell sorting, etc.). We tailor and execute custom solutions to address every specific requirement. With numerous successful cases in anti-ion channel antibody production, anti-GPCR antibody production, and anti-transporter antibody production, our scientists are happy to work with you step by step to ensure the most satisfactory outcome. Please feel free to contact us for more details.