Empowered by leading technology platform and extensive experience in antibody development, Creative Biolabs is proud to offer our worldwide clients anti-membrane protein antibody discovery service using phage display immune library in rabbit.
Membrane proteins play various roles in cellular physiological functions and activities. They constitute the targets of over 50% drugs/therapies in market. Nowadays, anti-membrane protein antibody becomes more and more popular in therapeutic field due to higher specificity, higher affinity compared to small molecular drugs. Therefore, discovery of anti-membrane protein antibody has been a constant hotspot for both research and therapeutic purposes. Equipped with world-class phage display platform, Creative Biolabs is professional in ideal anti-membrane protein antibodies via phage display rabbit library construction and screening strategy.
Phage display technology, which can physically link phenotype with genotype, allows efficient and comprehensive screenings for adequate clones in vast antibody libraries. Being one of the most effective ways for anti-membrane protein antibody discovery without species limitation, rabbit phage display immune library technique exerts many unique features including:
Rabbits belonging to the order Lagomorpha, are evolutionarily distant from mice and rats. Epitopes conserved between rodent and human antigens that are invisible in rodents can often be recognized by rabbit immune systems.
Due to higher sensitivity of immune system, antibodies produced by rabbits normally have 10 to 100-fold higher affinity for antigen than mouse monoclonal antibodies. Therefore, rabbit antibody repertoire is an exceptional source for both polyclonal antibodies and monoclonal antibodies.
The rabbit immune system can generate antibodies that are able to distinguish between very similar molecules with subtle structural variations.
Creative Biolabs has a well-established platform for rabbit phage display immune library construction and screening. Combined with intrinsic advantages of phage display technique (high throughput, high efficient, large candidate pool), we have successfully tailored numerous rabbit mAb against a variety of membrane protein targets. Usually, two or more rabbits of the b9 allotype are immunized with one antigen, total RNA from spleen lymphocytes and bone marrow cells are purified, and then chimeric rabbit/human Fab libraries are made in our pCDisplay phagemid vectors by fusing rabbit Vκ/Vλ to human Cκ and rabbit VH to human CH1 of human IgG1. Multiple screening strategies can be applied, e.g. solution-sorting screening, solid phase screening, cell-based screening, etc.
Of note, we provide one-stop anti-membrane protein antibody discovery service including immunogen design. As is known, membrane proteins with adequate conformation & functionalities can be very challenging to obtain. Based on our all-round Magic™ membrane protein immunogen preparation platform, our scientists are specialized in tailoring different antigen formats (e.g. different expression systems, different stabilization options) according to each specific target. Flexible options can be offered to fulfill your particular needs.
Creative Biolabs is constantly committed to assisting our global clients with their anti-membrane protein antibody discovery projects. In addition to rabbit phage display library approach, we also provide multiple strategies for your selection (e.g. hybridoma, B cell sorting, etc.). With numerous successful cases in anti-ion channel antibody production, anti-GPCR antibody production, and anti-transporter antibody production, we are more than happy to leverage our knowledge and technologies to address custom demands. Please feel free to contact us for more details.