Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications. Highlights
Creative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
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HighlightsEmpowered by state-of-the-art technologies and equipment, Creative Biolabs now offers world-leading anti-membrane protein antibody production service using polymers. Membrane proteins incorporated into polymers show attractive characteristics in animal immunization and antibody selection to obtain desired binders.
One of the main limitations for anti-membrane protein antibody discovery lies in the antigen preparation step. As is known, the low natural abundance, complicated structures, large hydrophobic regions, unstable conformation in solutions, etc., make it very challenging to obtain membrane proteins in proper structural and functional state. In order to overcome these difficulties, Creative Biolabs has set up a Magic™ Platform to produce membrane proteins using polymers. Currently, we can offer two amphipathic polymer solutions for membrane proteins (purified or unpurified) to our clients all over the world.
Introduction of Polymers
The first widely used amphipathic polymer is amphipols (e.g. A8-35). They can trap the transmembrane region(s) of target protein, thus keeping the hydrophobic domains stay folded. Meanwhile, the biological functions of membrane proteins will not be changed. It is relatively easy to transfer membrane proteins from micelles to amphipols. By adding amphipols into the micellar solution at neutral or alkaline pH, followed by removing the detergent components, membrane proteins will be wrapped in amphipols. They have been successfully applied for a lot of membrane proteins, including GPCRs. Poly (styrene-co-maleic acid) lipid particles is another kind of low-molecular-weight amphipathic polymer. They can be used in a detergent-free manner. When the copolymers are added to membrane environment at pH 7 to 8, uniform particles are formed after membrane protein solubilization. Then standard purification methods can be used to purify membrane proteins in polymer particles. For different proteins, the diameters would vary between 10 and 24 nm.
Advantages of Polymers
Polymers have high solubility to keep membrane proteins soluble in aqueous solutions. Besides, membrane proteins incorporated into polymers will be more stable in this native-like environment. Moreover, SMA copolymer can directly extract and stabilize membrane proteins from the cell membrane, no need for risky detergent solubilization process. Notably, their abilities to stabilized membrane proteins are very flexible, not restricted by membrane protein size, secondary structure, aggregation state or distribution of hydrophilic and hydrophobic surfaces. Therefore, membrane proteins in polymers can serve as excellent antigens for antibody development.
Features of Membrane Protein Immunogen Preparation in Polymers
Creative Biolabs has gained rich experience in membrane protein immunogen preparation. We now provide one-stop services from initial immunogen design & preparation to antibody discovery and engineering. Particularly, based on our versatile Magic™ Platform, we also have following recombinant membrane protein preparation options for your consideration:
So far we have successfully generated many GPCR immunogens, ion channel immunogens, transporter immunogens for our customers. Please feel free to contact us for more information.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
