Empowered by state-of-the-art technologies and equipment, Creative Biolabs now offers world-leading anti-membrane protein antibody production service using polymers. Membrane proteins incorporated into polymers show attractive characteristics in animal immunization and antibody selection to obtain desired binders.
One of the main limitations for anti-membrane protein antibody discovery lies in the antigen preparation step. As is known, the low natural abundance, complicated structures, large hydrophobic regions, unstable conformation in solutions, etc., make it very challenging to obtain membrane proteins in proper structural and functional state. In order to overcome these difficulties, Creative Biolabs has set up a Magic™ Platform to produce membrane proteins using polymers. Currently, we can offer two amphipathic polymer solutions for membrane proteins (purified or unpurified) to our clients all over the world.
Introduction of Polymers
The first widely used amphipathic polymer is amphipols (e.g. A8-35). They can trap the transmembrane region(s) of target protein, thus keeping the hydrophobic domains stay folded. Meanwhile, the biological functions of membrane proteins will not be changed. It is relatively easy to transfer membrane proteins from micelles to amphipols. By adding amphipols into the micellar solution at neutral or alkaline pH, followed by removing the detergent components, membrane proteins will be wrapped in amphipols. They have been successfully applied for a lot of membrane proteins, including GPCRs. Poly (styrene-co-maleic acid) lipid particles (SMALPs) is another kind of low-molecular-weight amphipathic polymer. They can be used in a detergent-free manner. When SMA copolymers are added to membrane environment at pH 7 to 8, uniform SMALPs are formed after membrane proteins solubilization. Then standard purification methods can be used to purify membrane proteins in SMALPs. For different proteins, the diameters of SMALPs would vary between 10 and 24 nm.
Advantages of Polymers
Polymers have high solubility to keep membrane proteins soluble in aqueous solutions. Besides, membrane proteins incorporated into polymers will be more stable in this native-like environment. Moreover, SMA copolymer can directly extract and stabilize membrane proteins from the cell membrane, no need for risky detergent solubilization process. Notably, their abilities to stabilized membrane proteins are very flexible, not restricted by membrane protein size, secondary structure, aggregation state or distribution of hydrophilic and hydrophobic surfaces. Therefore, membrane proteins in polymers can serve as excellent antigens for antibody development.
Features of Membrane Protein Immunogen Preparation in Polymers
Creative Biolabs has gained rich experience in membrane protein immunogen preparation. We now provide one-stop services from initial immunogen design & preparation to antibody discovery and engineering. Particularly, based on our versatile Magic™ Platform, we also have following recombinant membrane protein preparation options for your consideration: