Anti-Membrane Protein Antibody Production Using Recombinant Proteins
With years of experience in membrane protein production and antibody discovery, Creative Biolabs has gained significant knowledge in anti-membrane protein antibody production. Equipped with our first-class expression systems, we are confident to help our customers prepare recombinant membrane proteins in various formats to meet each specific demands.
Membrane proteins exert their cellular functions as receptors, transporters, channels, etc. They have long been attractive drug targets for treatment of various diseases. In recent years, anti-membrane protein antibody has been drawing increasing research interests due to their higher specificity and lower cytotoxicity. A vital part of antibody discovery is antigen preparation for immunization and binder selection. However, it is quite challenging to generate recombinant membrane proteins because they are highly hydrophobic with complicated structure and low natural abundance. To get rid of the situation, Creative Biolabs offers this customized service of tailoring functional recombinant membrane protein based on your timeline and budget.
Recombinant expression is currently the conventional and frequently applied approach for membrane protein production. Based on our well-established cell-based expression and cell-free expression systems from prokaryotic and eukaryotic systems, we have accumulated valuable experience in membrane protein expression, solubilization, purification, and characterization from small to large scales.
Anti-Membrane Protein Antibody Production Using Proteoliposomes: proteoliposomes are one of the most frequently used methods for membrane protein reconstitution, which can suit many applications. Proteoliposomes have advantage to keep membrane proteins in their native-like environment. Based on our Lipid Screening Platform, we can perform comprehensive screening to find the most appropriate lipid composition for each particular target. We are professional in proteoliposome preparation from various starting materials, such as intact cells, membrane crude extracts, and membrane proteins already solubilized or reconstituted in micelles, bicelles, nanodiscs, amphipols or other liposomes.
Anti-Membrane Protein Antibody Production Using Lipoparticles: lipoparticles are highly purified virus-like particles (VLPs) which carry intact, native membrane fractions of host cells on the outer surface. A key step for high-quality lipoparticle production is the proper choice of appropriate expression host system. E. coli, yeast cell, insect cell, mammalian cell and plant systems are all well-established host systems in Creative Biolabs. This option is particularly suitable for immunization use. In addition, we also provide many well-characterized VLP products.
Anti-Membrane Protein Antibody Production Using Nanodiscs: nanodisc is one of the best mimics of native environment for membrane proteins, consisting of a mixture of lipids and membrane scaffolding protein (MSP). They are more stable, homologous, controllable, and compatible with a variety of expression systems. It can be used for a wide range of applications such as antibody discovery, conformation dynamics, functional detections, etc.
Anti-Membrane Protein Antibody Production Using Polymers: there are two major types of polymers, amphipols, and poly (styrene-co-maleic acid) lipid particles (SMALPs). These amphipathic molecules increase the potency of right-folded target proteins with higher stability. Meanwhile, polymers can be employed to perform membrane protein preparation in a detergent-free manner.
Anti-Membrane Protein Antibody Production Using Designed Peptides: well-designed peptides can act as good surrogates of full-length membrane proteins. They are usually derived from extracellular or intracellular loops of target proteins with similar immunogenicity and characteristics but with increased stability and solubility. No need for the time-consuming expression, purification and reconstitution process, designed peptides can be easily obtained from chemical synthesis in less than 3 weeks.
Till now, Creative Biolabs has successfully obtained hundreds of functional GPCR immunogens, ion channel immunogens, and transporter immunogens, constituting solid foundation for successful antibody development campaign. We are more than happy to share our experience and offer à la carte services customized to meet your special needs. Please feel free to contact us for more details.