Creative Biolabs is a leading service provider focus on antibody production. Based on our extensive experience and professional scientists, we have applied the conventional immunization method to achieve hundreds of novel antibody discovery projects in varies of animal species. We are proud to offer the unique service for our customers all over the world to free up your time for core work and project.
What Is Conventional Immunization?
Conventional immunization is the essential immunization method that is widely used in laboratories. The antigens for conventional immunization can be proteins, peptides, organelles, cells, and tissues. The specific immunization method can be, among the others, subcutaneous injection, intraperitoneal injection, intravenous injection, and intramuscular injection.
Subcutaneous injection refers to the liquid into the subcutaneous tissue. It is highly effective in administering vaccines and medications. The absorption rate of subcutaneous injection is faster than intradermal injection but slower than intramuscular injection as there are few blood vessels in subcutaneous tissues. Subcutaneous injection is the easiest injection technique which can also avoid the anaphylactic reactions occurring induced by the introduction of antigen in blood vessels. However, the antigen requirement is always large.
Intramuscular injection is one of the alternative methods for the injection of a substance directly into muscle. Compared with the subcutaneous tissue, muscles have more and larger blood vessels which provide the faster absorption rate. Depending on the injection site, the fluid injected is always limited between 2 and 5 milliliters. The possible sites for intramuscular injection include rectus femoris, deltoid, dorsogluteal, ventrogluteal muscles, and vastus lateralis. The advantage of intramuscular injection is liquid absorption and easy operation.
Intraperitoneal injection is the injection of a substance into the peritoneum. This is an easy and convenient injection technique for animals, especially for rodents. Moreover, in this way the antigen can be detected by lymphatic system quickly and spread widely to lymphoid tissue.
Intravenous injection is a sensible choice when it is utilized for booster injections following the primary immunization. This method requires operator injecting the antigen into a vein carefully. What’ more, this method is good for the antigens without adjuvants as the antigen-adjuvant emulsion is large enough to form embolism blocking blood vessels. However, the antigen effects of this approach cannot last long. As a result, the recipients are more likely to have tolerance and fail to induce immune responses.
Fig.1 Different injection methods.
Creative Biolabs is one of the well-recognized experts who are professional in applying varies of immunization strategies for a broad range of antibodies production. We are therefore confident in offering the best and most suitable service for our customers all over the world. If you are interested in our service, please do not hesitate to inquire us for more details.