Arginylation Specific Antibody Discovery Service

Background Antibody Types Discovery Strategy Types of PTM Why Choose Us? FAQs

Creative Biolabs provides Arginylation-Specific Antibody Discovery Services to help researchers overcome difficulties in protein arginylation measurement, as well as in developing precise antibodies and acquiring dependable research instruments for protein functionality studies. We solve these problems by providing high-affinity antibodies that specifically target arginylation. These services utilize state-of-the-art antibody development platforms and novel techniques to provide complete solutions for arginylation challenges.

Background

Arginylation represents a post-translational modification (PTM) through which arginine attaches to proteins, mainly β-actin in mammalian cells. The arginyltransferase enzyme (ATE1) drives a vital process that supports several cellular functions by enabling cell migration and contractility while facilitating cell adhesion. Studies show that arginylation plays an essential role in maintaining cell leading edge stability and directing cell migration speed and direction while supporting neurite development.

Fig. 1 Antibody generation and validation (OA Literature). Fig.1 Peptide mixtures used for antibody generation and validation.¹

N-terminal arginylation of actin is present in evolutionarily simpler organisms such as Dictyostelium discoideum and its absence leads to abnormalities in the actin cytoskeleton, together with substrate adhesion and cell movement. The selective regulation of actin isoforms through N-terminal arginylation demonstrates conservation as a mechanism throughout eukaryotic species. Detection of arginylated actin within cells remains difficult because intracellular actin normally undergoes N-terminal acetylation, which prevents arginylation. The technical difficulties present have limited researchers' capacity to examine the distribution and presence of N-terminal actin arginylation in different cells and tissues. The importance of services provided by Creative Biolabs becomes evident because arginylation plays a pivotal role in basic biological functions, while researchers face significant obstacles in studying and detecting it.

Antibody Types

Creative Biolabs provides monoclonal and polyclonal antibodies with hosts including Rabbit, Mouse, and Sheep. Our antibody products include:

Site-Specific Anti-Arginylation Antibodies: These antibodies attach to a precise arginylation site located on a protein molecule. These antibodies focus on binding to specific amino acid residues which have undergone arginylation modification.
Pan Anti-Arginylation Antibodies: These antibodies recognize arginylation modifications across all amino acid sequences. These antibodies detect arginylation modifications on proteins without regard to their specific location.

Discovery Strategy

Creative Biolabs utilizes three primary methods to develop antibodies.

Monospecific anti-arginylation polyclonal antibody production: KLH-conjugated synthetic arginylation-modified peptides in optimized immunization protocols generate polyclonal antibodies that identify arginylated proteins.

Fig. 2 General process of monospecific anti-arginylation polyclonal antibody production. (Creative Biolabs Original)

Phage display strategy for anti-arginylation monoclonal antibody discovery: Phage display technology allows scientists to identify monoclonal antibodies which exhibit strong binding affinity for arginylated proteins through selection and identification processes.

Fig. 3 General process of phage display for anti-arginylation monoclonal antibody discovery. (Creative Biolabs Original)

Hybridoma strategy for anti-arginylation monoclonal antibody discovery: The traditional approach requires animal immunization before the fusion of their spleen cells with myeloma cells to produce hybridomas that create monoclonal antibodies against arginylated proteins.

Fig. 4 General process of hybridoma for anti- arginylation monoclonal antibody discovery. (Creative Biolabs Original)

Types of PTM

We provide arginylation specific antibodies and antibodies targeting a variety of PTMs, as shown in the table below.

Why Choose Us?

You can count on Creative Biolabs as your prime partner for Arginylation-Specific Antibody Discovery Services because of our:

High Specificity and Affinity: Our team delivers high-affinity antibodies that exclusively bind to arginylated proteins to guarantee precise and dependable outcomes.
Comprehensive Service Portfolio: Creative Biolabs specializes in producing PTM-specific antibodies through its extensive service offerings, which reflect our expertise in developing diverse antibody solutions.
Technical Expertise: Through our advanced antibody development platforms and innovative techniques. Our antibody-based techniques advance arginylation research.
Commitment to Quality: Creative Biolabs maintains strict quality control processes to guarantee their production of superior antibodies.

FAQs

Q: What are Arginylation-Specific Antibodies used for?

A: Arginylation-specific antibodies serve as crucial research tools to investigate protein arginylation, which plays a role in multiple cellular processes. Researchers can use these antibodies to detect arginylated proteins while analyzing their involvement in biological processes and disease mechanisms. Reach out to our team to learn about tailored applications that can benefit your research.

Q: What are the differences between Arginylation-Specific Antibodies and other protein arginylation detection methods?

A: Antibodies provide a more specific and generally more sensitive detection method for arginylated proteins in complex biological samples compared to mass spectrometry, which identifies arginylation sites. Our team stands ready to guide you in selecting the optimal method that meets your specific research requirements.

Q: How do your arginylation-specific antibodies perform?

A: Creative Biolabs produces high-affinity antibodies that have been thoroughly characterized through optimized production protocols. Published data demonstrate how our approach ensures reliable and reproducible results. Contact us to receive detailed performance data and learn about the benefits of our antibodies for your research.

Q: What safety measures should I follow when working with Arginylation-Specific Antibodies?

A: All antibodies demand careful handling and storage along with validation to maintain their effectiveness. Our technical support team will deliver precise protocols and recommendations tailored to your application needs. Inquire now for expert guidance.

Q: Does Creative Biolabs provide customized Arginylation-Specific Antibodies to meet my research requirements?

A: Yes, Creative Biolabs offers customized solutions. Our antibody production services can be customized to fulfill your specific research needs. Reach out to our team to explore how we can create the ideal antibody solution for your research project.

Based on our advanced platforms, Creative Biolabs provides arginylation-specific antibody solutions to help speed up your research progress. If you are interested in our services, please do not hesitate to contact us for more details.

Reference

MacTaggart, Brittany, et al. "Global analysis of post-translational side-chain arginylation using pan-arginylation antibodies." Molecular & Cellular Proteomics 22.11 (2023). Distributed under Open Access license CC BY 4.0, without modification.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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