ASIC3 Membrane Protein Introduction

Introduction of ASIC3

ASIC3, also known as amiloride-sensitive cation channel 3 (ACCN3) or testis sodium channel 1 (TNaC1), is encoded by ASIC3 gene. It belongs to the ASIC/ENaC superfamily and is predominantly expressed in dorsal root ganglia neurons, especially in nociceptive sensory neurons. Recent studies show that ASIC3 plays an important role in pain perception, especially in high-intensity pain stimulation and acid-induced hyperalgesia. Meanwhile, ASIC3 has been regarded as a potential therapeutic strategy for arthritis.

Basic Information of ASIC3
Protein Name Acid-sensing ion channel 3
Gene Name ASIC3
Aliases ACCN3, SLNAC1, TNAC1
Organism Homo sapiens (Human)
UniProt ID Q9UHC3
Transmembrane Times 2
Length (aa) 531

Function of ASIC3 Membrane Protein

Acid-sensing ion channel 3 (ASIC3) has two hydrophobic transmembrane domains (TM1 and TM2), short intracellular N-and C- termini, and a large cysteine-rich extracellular loop. ASIC3 is extracellular proton-gated cation channel that has been implicated in multiple physiological and pathological processes. ASIC3 has also been shown to play an inhibitory role in synovial proliferation and subsequent accumulation of inflammatory mediators, indicative of a protective effect in joints. Researches reveal that acid sensing ion channel 3 (ASIC3) is the most sensitive to extracellular pH and has the characteristic ability to generate a biphasic current. Acid-sensing ion channel acid-sensing ion channel 3 (ASIC3) has been proposed to be involved in the pathophysiology of hypertension.

Model of the interaction of NHERF-1 with ASIC3. Fig.1 Model of the interaction of NHERF-1 with ASIC3. (Deval, 2006)

Application of ASIC3 Membrane Protein in Literature

  1. Yan X.G., et al. Subtype-selective inhibition of acid-sensing ion channel 3 by a natural flavonoid. CNS Neurosci Ther. 2018. PubMed ID: 29781252

    This article analyzes the effect of several compounds on the ASIC-mediated currents and acid-induced nocifensive behavior. These results show that small molecule drug can be designed to target ASIC3 channels to treat pain-related diseases.

  2. Zheng M.X., et al. Expression of Nerve Growth Factor and Type 3 of Acid Sensitive Ion Channels in Rat Model of Type Ⅲ Prostatitis. Sichuan Da Xue Xue Bao Yi Xue Ban. 2018, 49(1): 39-43. PubMed ID: 29737087

    Authors assess the expression levels of nerve growth factor (NGF) and acid-sensing ion channel 3 (ASIC3) in prostatic tissue of experimental rats with type Ⅲ prostatitis. The data illustrate that NGF and ASIC3 are highly expressed in the prostate with EAP.

  3. Cao Q., et al. Inhibition of Acid Sensing Ion Channel 3 Aggravates Seizures by Regulating NMDAR Function. Neurochem Res. 2018. PubMed ID: 29736613

    Authors in this group reveal that ASIC3 expression is increased in the hippocampus of pilocarpine induced seizure rats, as well as in hippocampal neuronal cultures undergoing epileptiform discharge elicited by Mg2+-free media and find ASIC3 agonists could be used to seizure treatment.

  4. Er L.K., et al. Combined effect of acid-sensing ion channel 3 and transient receptor potential vanilloid 1 gene polymorphisms on blood pressure variations in Taiwanese. Ci Ji Yi Xue Za Zhi. 2018, 30(1): 29-36. PubMed ID: 29643714

    Authors analyze the genetic polymorphisms of the ASIC3 and TRPV1 genes and blood pressure (BP) variations in Taiwanese population. These results indicate that the interaction between ASIC3 and TRPV1 is involved in BP regulation.

  5. Stephan G., et al. The ASIC3/P2X3 cognate receptor is a pain-relevant and ligand-gated cationic channel. Nat Commun. 2018, 9(1): 1354. PubMed ID: 29636447

    This article reveals the interaction relationship between acid-sensing ion channels (ASIC3) and of ATP-sensitive P2X receptors (P2X3Rs) in sensory neurons. The receptor sub-units are tightly associate with each other to form a protein complex, mediating unidirectional inhibition.

ASIC3 Preparation Options

To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-ASIC3 antibody development services.

As a forward-looking research institute as well as a leading custom service provider in the field of membrane protein, Creative Biolabs has won good reputation among our worldwide customers for successfully accomplishing numerous challenging projects including generation of many functional membrane proteins. Please feel free to contact us for more information.


  1. Deval E, et al. (2006). Regulation of sensory neuron-specific acid-sensing ion channel 3 by the adaptor protein Na+/H+ exchanger regulatory factor-1. J Biol Chem. 281(3): 1796-807.

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