Introduction of ASIC4
ASIC4, also known as amiloride-sensitive cation channel 4 (ACCN4), is encoded by ASIC4 gene. It belongs to the ASIC/ENaC superfamily, and is predominantly distributed in mammalian nervous systems as well as non-neural tissues, such as cancer cells, articular chondrocytes, and intervertebral disc cells. Recent studies show ASIC4 plays crucial roles in modulating cell behavior via regulation of intracellular Ca2+ accumulation, including apoptosis, differentiation, and autophagy. Meanwhile, ASIC4 has been a novel pharmacological and therapeutic target for degenerative diseases.
|Basic Information of ASIC4|
|Protein Name||Acid-sensing ion channel 4|
|Organism||Homo sapiens (Human)|
Function of ASIC4 Membrane Protein
Acid-sensing ion channel 4 (ASIC4) has two hydrophobic transmembrane domains (TM1 and TM2), short intracellular N-and C- termini, and a large cysteine-rich extracellular loop. ASIC4 usually exists within inner ear neurons, adenohypophysis, and intervertebral disc. Multiple lines of evidence suggest that aberrant expression and activation of ASICs contribute to the progression of various degenerative diseases, including multiple sclerosis, Parkinson's disease, Huntington's disease, intervertebral disc degeneration and arthritis.
Fig.1 Closed (desensitized) rat ASIC4 model. (Sazanavets, 2015)
Application of ASIC4 Membrane Protein in Literature
This article analyzes the expression level of ASIC4 in the adult wild-type mouse brain. The result shows the ASIC4 mRNAs are widely expressed throughout the wild-type brain, but preferentially concentrate in several areas, which indicates ASIC4 may be related with some brain diseases.
Authors analyze the subcellular localization of ASIC4 in heterologous cell lines, which reveal a surprising accumulation of the channel in early endosome-related vacuoles. The data illustrate that heterologously expressed ASIC4 predominantly resides in an intracellular endosomal compartment.
Authors in this group reveal that ASIC4 expression and physiological function on mice. The data suggest that ASIC4 might play an important role in modulating the innate fear response to predator odor and anxious state.
ASIC4 Preparation Options
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