This product is a Raji B cell engineering tool based on the Gold nanoparticles (N-acetyl-D-glucosamine,sialic acid/Wheat germagglutinin) delivery system and CRISPR-based (SHS-229-huIgH296-Cpf1-3'-Azido-3'-deoxythymidine ) genome editor.
| Host Cell | Raji |
| Target | 5T4 |
| Insert Clone | 5G4H10G5 |
| Safe Harbor Sites (SHS) | SHS-229 |
| gRNA | huIgH296 |
| Nuclease | Cpf1 |
| Enhancer | 3'-Azido-3'-deoxythymidine |
| Targeting Strategy | pH responsive, time-dependent |
| Insertion sites | Mouse genome E-7 |
| Vehicles | Gold nanoparticles |
| Ligand/Receptor | N-acetyl-D-glucosamine, sialic acid/Wheat germ agglutinin |
| Active Agents | Thymoquinone |
| Particle Size | 1-100 |
| Charge (mv) | <-25 |
| Total Atom Count | ~20,000,000 |
| Surface Atoms (%) | ~4 |
| Specific Surface Area (m2/g) | ~8 |
| Vector Type | VSV |
| Vector Name | BCpCDH7 |
| Targeting Diseases | Breast cancer; Lung cancer |
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