Creative Biolabs is dedicated to providing B cell engineering services by CRISPR/Cas9-based gene editing at the Rosa26 locus. The safe integration ensures transcription-competency of the transgenes and presents no known adverse effects on fitness.
Creative Biolabs has an experienced team of experts and a mature technology platform for producing drug-secreting B cells. We have developed a comprehensive ROSA26 CRISPR service to help researchers insert their gene of interest into the ROSA26 genomic locus of B cells through homologous recombination. Our experts have accumulated a large amount of data on biological information and gene editing which have allowed us to offer designing, construction, and preparation of vectors for the generation of genetically engineered B cells.
A CRISPR-CAS9 construct to target the ROSA26 safe-harbor locus includes:
Fig.1 CRISPR/Cas9-based genomic editing at the Rosa26 Locus. (Creative Biolabs)
B cells are an ideal gene therapy target cell choice for secreted therapeutic proteins because of their ease of isolation from peripheral blood, their ability to expand in vitro, and their longevity in vivo. Creative Biolabs is supporting clients who are developing engineered B cells using our expertise in genome editing, B cell biology, immunology, and engineered cell therapy research. We are using our technologies and experience to develop B cells that express protective antibodies and therapeutic protein. We also provide further functional assessment services for engineered B cells. Outlined below are the services we offer as part of our custom B cell engineering development service.
Fig.2 Experimental workflow of B cell engineering by CRISPR/Cas9. (Creative Biolabs)
B cells can be used as a strategy for delivering therapeutic proteins in vivo and their longevity gives them a lot of leverage as a cell therapeutic. Our B cell engineering strategy is harnessing a B cell to express various proteins with therapeutic potential, including:
The CRISPR/Cas9 gene-editing platform of Creative Biolabs is optimized for customized B cell development. Please feel free to contact us to learn how we help you accelerate your project needs.