RTN4R mAb (mATK63) B Cell Engineering Specific Plasmid (CAT#: BGS-1223-CR1)

This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GFP-UBC-Sec/SPII-CD79α-CD28-CBP) and CRISPR-Cas (NmCas9-ssRNA-HD/Cas3-5'PFS: non-C; 3'PFS:NAN/NNA-Stat5b) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.

Datasheet

Specifications

Clone mATK63
Host Mouse
Targeting Cell Type RPMI 1788
Reporter gene GFP
Promoter UBC
Signal peptides Sec/SPII
Signaling domains CD79α
TM domains CD28
Detection tags CBP
Linkers G4S linker
Co-Receptors CD22
Vector Type Adenoviruses
Vector Name BGpCDH3
Class 1 multi-Cas
Type I
Subtype A, B, C, D, E, F
Effector NmCas9
DNA Type ssRNA
Nuclease domains HD/Cas3
TracrRNA requirement No
PAM/PFS 5'PFS: non-C; 3'PFS:NAN/NNA
Insertion Stat5b
Construction GFP-UBC-CD79α-NmCas9-ssRNA-Stat5b

Target Information

Target RTN4R
Alternative Names RTN4R; NGR; NOGOR; Reticulon 4 receptor

Product Properties

Format Liquid/lyophilized
Shipping It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs.
Handling Advice No special measures are necessary. Keep away from food, drink and animal feedingstuffs.
Storage Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C.
COA

Certificate of Analysis Lookup

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