This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GFP-UBC-Sec/SPII-CD79α-CD28-CBP) and CRISPR-Cas (NmCas9-ssRNA-HD/Cas3-5'PFS: non-C; 3'PFS:NAN/NNA-Stat5b) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | mATK63 |
Host | Mouse |
Targeting Cell Type | RPMI 1788 |
Reporter gene | GFP |
Promoter | UBC |
Signal peptides | Sec/SPII |
Signaling domains | CD79α |
TM domains | CD28 |
Detection tags | CBP |
Linkers | G4S linker |
Co-Receptors | CD22 |
Vector Type | Adenoviruses |
Vector Name | BGpCDH3 |
Class | 1 multi-Cas |
Type | I |
Subtype | A, B, C, D, E, F |
Effector | NmCas9 |
DNA Type | ssRNA |
Nuclease domains | HD/Cas3 |
TracrRNA requirement | No |
PAM/PFS | 5'PFS: non-C; 3'PFS:NAN/NNA |
Insertion | Stat5b |
Construction | GFP-UBC-CD79α-NmCas9-ssRNA-Stat5b |
Target | RTN4R |
Alternative Names | RTN4R; NGR; NOGOR; Reticulon 4 receptor |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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