This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GFP-CMV-Tat/SPI-CD79α-CD28-His) and CRISPR-Cas (SaCas9-dsDNA-HD/Cas3-Stat5a) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | 7UL5 |
Host | Mouse |
Targeting Cell Type | Raji |
Reporter gene | GFP |
Promoter | CMV |
Signal peptides | Tat/SPI |
Signaling domains | CD79α |
TM domains | CD28 |
Detection tags | His |
Linkers | Thioredoxin with polylinker |
Co-Receptors | CD22 |
Vector Type | RABV |
Vector Name | BGpCDH8 |
Class | 2 single-Cas |
Type | Ⅴ |
Subtype | A, B, C, D, E, F, U |
Effector | SaCas9 |
DNA Type | dsDNA |
Nuclease domains | HD/Cas3 |
TracrRNA requirement | YES |
PAM/PFS | - |
Insertion | Stat5a |
Construction | GFP-CMV-CD79α-SaCas9-dsDNA-Stat5a |
Target | RUNX1 |
Alternative Names | RXFP1; LGR7; RXFPR1; Relaxin/insulin-like family peptide receptor 1; relaxin/insulin like family peptide receptor 1; relaxin family peptide receptor 1 |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
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