This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GFP-UBC-Sec/SPI-CD79α-CD8-CBP) and CRISPR-Cas (Cas12a (Cpf1)-ssRNA-NNGRRT-Wap) systems.
| Clone | 33DA9 |
| Host | Mouse |
| Targeting Cell Type | Raji |
| Reporter gene | RFP |
| Promoter | MNDU3 |
| Signal peptides | Sec/SPI |
| Signaling domains | CD79α |
| TM domains | CD8 |
| Detection tags | CBP |
| Linkers | SEG |
| Co-Receptors | LILRB |
| Vector Type | Baculovirus-AAV |
| Vector Name | BGpCDH4 |
| Class | 2 single-Cas |
| Type | III |
| Subtype | B |
| Effector | Cas12a (Cpf1) |
| DNA Type | ssRNA |
| Nuclease domains | Unknown |
| TracrRNA requirement | YES |
| PAM/PFS | NNGRRT |
| Insertion | Wap |
| Construction | RFP-MNDU3-CD79α-Cas12a (Cpf1)-ssRNA-Wap |
| Target | RXFP1 |
| Alternative Names | RXFP1; LGR7; RXFPR1; Relaxin/insulin-like family peptide receptor 1; relaxin/insulin like family peptide receptor 1; relaxin family peptide receptor 1 |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
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