RYK mAb (1671UET575.42.61) B Cell Engineering Specific Plasmid (CAT#: BGS-1223-CR17)

This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GUS-CMV+intron-Sec/SPIII-CD79α-CD28-Flag) and CRISPR-Cas (Cas12c (C2c3)-ssRNA-HD/Cas10-5' AT-rich PAM-ROSA26) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.

Datasheet

Specifications

Clone 1671UET575.42.61
Host Mouse
Targeting Cell Type NCI-H929 [H929]
Reporter gene GUS
Promoter CMV+intron
Signal peptides Sec/SPIII
Signaling domains CD79α
TM domains CD28
Detection tags Flag
Linkers G4S linker
Co-Receptors CD72
Vector Type Adenoviruses
Vector Name BGpCDH3
Class 1 multi-Cas
Type I
Subtype A
Effector Cas12c (C2c3)
DNA Type ssRNA
Nuclease domains HD/Cas10
TracrRNA requirement YES
PAM/PFS 5' AT-rich PAM
Insertion ROSA26
Construction GUS-CMV+intron-CD79α-Cas12c (C2c3)-ssRNA-ROSA26

Target Information

Target RYK
Alternative Names RYK; D3S3195; JTK5; JTK5A; RYK1; receptor-like tyrosine kinase; receptor like tyrosine kinase

Product Properties

Format Liquid/lyophilized
Shipping It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs.
Handling Advice No special measures are necessary. Keep away from food, drink and animal feedingstuffs.
Storage Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C.
COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number
! For Research Use Only | Not For Clinical Use

Related products