This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (Luc-EFS-Sec/SPI-CD79β-CD28-CBP) and CRISPR-Cas (Cas13a (C2c2)-ssRNA-2xHEPN-NNNNGATT-attP) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | BGPIUENR-939 |
Host | Rabbit |
Targeting Cell Type | Raji-Luc2 |
Reporter gene | Luc |
Promoter | EFS |
Signal peptides | Sec/SPI |
Signaling domains | CD79β |
TM domains | CD28 |
Detection tags | CBP |
Linkers | Glycine-Serine linker |
Co-Receptors | Siglec-G |
Vector Type | VSV |
Vector Name | BGpCDH7 |
Class | 1 multi-Cas |
Type | IV |
Subtype | A, B |
Effector | Cas13a (C2c2) |
DNA Type | ssRNA |
Nuclease domains | 2xHEPN |
TracrRNA requirement | No |
PAM/PFS | NNNNGATT |
Insertion | attP |
Construction | Luc-EFS-CD79β-Cas13a (C2c2)-ssRNA-attP |
Target | RYK |
Alternative Names | RYK; D3S3195; JTK5; JTK5A; RYK1; receptor-like tyrosine kinase; receptor like tyrosine kinase |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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