RYK mAb (UL75-P7-RU7-UL5) B Cell Engineering Specific Plasmid (CAT#: BGS-1223-CR12)

This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GUS-hPGK-Sec/SPII-CD79β-CD8-Flag) and CRISPR-Cas (Cas13b (C2c4)-dsDNA-HD/Cas10-5' AT-rich PAM-ROSA26) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.

Datasheet

Specifications

Clone UL75-P7-RU7-UL5
Host Mouse
Targeting Cell Type Ramos (RA 1)
Reporter gene GUS
Promoter hPGK
Signal peptides Sec/SPII
Signaling domains CD79β
TM domains CD8
Detection tags Flag
Linkers Glycine linker
Co-Receptors FcγRIIB
Vector Type Lentivirus
Vector Name BGpCDH2
Class 2 single-Cas
Type III
Subtype A, B, C
Effector Cas13b (C2c4)
DNA Type dsDNA
Nuclease domains HD/Cas10
TracrRNA requirement No
PAM/PFS 5' AT-rich PAM
Insertion ROSA26
Construction GUS-hPGK-CD79β-Cas13b (C2c4)-dsDNA-ROSA26

Target Information

Target RYK
Alternative Names RYK; D3S3195; JTK5; JTK5A; RYK1; receptor-like tyrosine kinase; receptor like tyrosine kinase

Product Properties

Format Liquid/lyophilized
Shipping It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs.
Handling Advice No special measures are necessary. Keep away from food, drink and animal feedingstuffs.
Storage Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C.
COA

Certificate of Analysis Lookup

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