This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GUS-hPGK-Sec/SPII-CD79β-CD8-Flag) and CRISPR-Cas (Cas13b (C2c4)-dsDNA-HD/Cas10-5' AT-rich PAM-ROSA26) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | UL75-P7-RU7-UL5 |
Host | Mouse |
Targeting Cell Type | Ramos (RA 1) |
Reporter gene | GUS |
Promoter | hPGK |
Signal peptides | Sec/SPII |
Signaling domains | CD79β |
TM domains | CD8 |
Detection tags | Flag |
Linkers | Glycine linker |
Co-Receptors | FcγRIIB |
Vector Type | Lentivirus |
Vector Name | BGpCDH2 |
Class | 2 single-Cas |
Type | III |
Subtype | A, B, C |
Effector | Cas13b (C2c4) |
DNA Type | dsDNA |
Nuclease domains | HD/Cas10 |
TracrRNA requirement | No |
PAM/PFS | 5' AT-rich PAM |
Insertion | ROSA26 |
Construction | GUS-hPGK-CD79β-Cas13b (C2c4)-dsDNA-ROSA26 |
Target | RYK |
Alternative Names | RYK; D3S3195; JTK5; JTK5A; RYK1; receptor-like tyrosine kinase; receptor like tyrosine kinase |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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