This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GUS-MNDU3-Sec/SPI-CD79β-CD28-GST) and CRISPR-Cas (FnCas9-RNA-Stat5a) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | S-133A1/1332 |
Host | Mouse |
Targeting Cell Type | MC116 |
Reporter gene | GUS |
Promoter | MNDU3 |
Signal peptides | Sec/SPI |
Signaling domains | CD79β |
TM domains | CD28 |
Detection tags | GST |
Linkers | Thioredoxin with polylinker |
Co-Receptors | CD81 |
Vector Type | MMLV |
Vector Name | BGpCDH5 |
Class | 1 multi-Cas |
Type | III |
Subtype | A, B, C, D, E, F |
Effector | FnCas9 |
DNA Type | RNA |
Nuclease domains | Unknown |
TracrRNA requirement | YES |
PAM/PFS | - |
Insertion | Stat5a |
Construction | GUS-MNDU3-CD79β-FnCas9-RNA-Stat5a |
Target | S100A1 |
Alternative Names | S100A1; S100; S100-alpha; S100A; S100 calcium-binding protein A1; S100 calcium binding protein A1 |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
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