S100A1 mAb (S-133A1/1942) B Cell Engineering Specific Plasmid (CAT#: BGS-1223-CR23)

This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (Luc-EFS-Sec/SPIII-CD79α-CD28-CBP) and CRISPR-Cas (Cas12a (Cpf1)-dsDNA-2xHEPN-Stat5a) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.

Datasheet

Specifications

Clone S-133A1/1942
Host Mouse
Targeting Cell Type SU-DHL-4
Reporter gene Luc
Promoter EFS
Signal peptides Sec/SPIII
Signaling domains CD79α
TM domains CD28
Detection tags CBP
Linkers 22 Amino Acid Linker
Co-Receptors Siglec-G
Vector Type AAV
Vector Name BGpCDH1
Class 1 multi-Cas
Type VI
Subtype A, B, C, D
Effector Cas12a (Cpf1)
DNA Type dsDNA
Nuclease domains 2xHEPN
TracrRNA requirement No
PAM/PFS -
Insertion Stat5a
Construction Luc-EFS-CD79α-Cas12a (Cpf1)-dsDNA-Stat5a

Target Information

Target S100A1
Alternative Names S100A1; S100; S100-alpha; S100A; S100 calcium-binding protein A1; S100 calcium binding protein A1

Product Properties

Format Liquid/lyophilized
Shipping It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs.
Handling Advice No special measures are necessary. Keep away from food, drink and animal feedingstuffs.
Storage Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C.
COA

Certificate of Analysis Lookup

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