This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (Luc-EFS-Sec/SPIII-CD79α-CD28-CBP) and CRISPR-Cas (Cas12a (Cpf1)-dsDNA-2xHEPN-Stat5a) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | S-133A1/1942 |
Host | Mouse |
Targeting Cell Type | SU-DHL-4 |
Reporter gene | Luc |
Promoter | EFS |
Signal peptides | Sec/SPIII |
Signaling domains | CD79α |
TM domains | CD28 |
Detection tags | CBP |
Linkers | 22 Amino Acid Linker |
Co-Receptors | Siglec-G |
Vector Type | AAV |
Vector Name | BGpCDH1 |
Class | 1 multi-Cas |
Type | VI |
Subtype | A, B, C, D |
Effector | Cas12a (Cpf1) |
DNA Type | dsDNA |
Nuclease domains | 2xHEPN |
TracrRNA requirement | No |
PAM/PFS | - |
Insertion | Stat5a |
Construction | Luc-EFS-CD79α-Cas12a (Cpf1)-dsDNA-Stat5a |
Target | S100A1 |
Alternative Names | S100A1; S100; S100-alpha; S100A; S100 calcium-binding protein A1; S100 calcium binding protein A1 |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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