S100A1 mAb (S-133A1/4436R) B Cell Engineering Specific Plasmid (CAT#: BGS-1223-CR20)

This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (RFP-mPGK-Sec/SPIII-CD79β-CD8-HA) and CRISPR-Cas (Cas12a (Cpf1)-dsDNA-HD/Cas3-NNNNGATT-Alb) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.

Datasheet

Specifications

Clone S-133A1/4436R
Host Rabbit
Targeting Cell Type Ramos (RA 1)
Reporter gene RFP
Promoter mPGK
Signal peptides Sec/SPIII
Signaling domains CD79β
TM domains CD8
Detection tags HA
Linkers Thioredoxin with polylinker
Co-Receptors CD19
Vector Type MSCV
Vector Name BGpCDH6
Class 2 single-Cas
Type III
Subtype A, B, C
Effector Cas12a (Cpf1)
DNA Type dsDNA
Nuclease domains HD/Cas3
TracrRNA requirement No
PAM/PFS NNNNGATT
Insertion Alb
Construction RFP-mPGK-CD79β-Cas12a (Cpf1)-dsDNA-Alb

Target Information

Target S100A1
Alternative Names S100A1; S100; S100-alpha; S100A; S100 calcium-binding protein A1; S100 calcium binding protein A1

Product Properties

Format Liquid/lyophilized
Shipping It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs.
Handling Advice No special measures are necessary. Keep away from food, drink and animal feedingstuffs.
Storage Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C.
COA

Certificate of Analysis Lookup

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