This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (RFP-mPGK-Sec/SPIII-CD79β-CD8-HA) and CRISPR-Cas (Cas12a (Cpf1)-dsDNA-HD/Cas3-NNNNGATT-Alb) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | S-133A1/4436R |
Host | Rabbit |
Targeting Cell Type | Ramos (RA 1) |
Reporter gene | RFP |
Promoter | mPGK |
Signal peptides | Sec/SPIII |
Signaling domains | CD79β |
TM domains | CD8 |
Detection tags | HA |
Linkers | Thioredoxin with polylinker |
Co-Receptors | CD19 |
Vector Type | MSCV |
Vector Name | BGpCDH6 |
Class | 2 single-Cas |
Type | III |
Subtype | A, B, C |
Effector | Cas12a (Cpf1) |
DNA Type | dsDNA |
Nuclease domains | HD/Cas3 |
TracrRNA requirement | No |
PAM/PFS | NNNNGATT |
Insertion | Alb |
Construction | RFP-mPGK-CD79β-Cas12a (Cpf1)-dsDNA-Alb |
Target | S100A1 |
Alternative Names | S100A1; S100; S100-alpha; S100A; S100 calcium-binding protein A1; S100 calcium binding protein A1 |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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