This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (CAT-EF1A-Sec/SPI-CD79β-CD28-CBP) and CRISPR-Cas (Cas13a (C2c2)-ssRNA-2xHEPN-NNNNGATT-attP) systems.
| Clone | 11P8.7 |
| Host | Human |
| Targeting Cell Type | Raji-Luc2 |
| Reporter gene | Luc |
| Promoter | EFS |
| Signal peptides | Sec/SPI |
| Signaling domains | CD79β |
| TM domains | CD28 |
| Detection tags | CBP |
| Linkers | Glycine-Serine linker |
| Co-Receptors | Siglec-G |
| Vector Type | VSV |
| Vector Name | BGpCDH7 |
| Class | 1 multi-Cas |
| Type | IV |
| Subtype | A, B |
| Effector | Cas13a (C2c2) |
| DNA Type | ssRNA |
| Nuclease domains | 2xHEPN |
| TracrRNA requirement | No |
| PAM/PFS | NNNNGATT |
| Insertion | attP |
| Construction | Luc-EFS-CD79β-Cas13a (C2c2)-ssRNA-attP |
| Target | S100A4 |
| Alternative Names | S100A4; 18A2; 42A; CAPL; FSP1; MTS1; P9KA; PEL98; S100 calcium binding protein A4 |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.