This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (YFP-EFS-Sec/SPII-CD79β-CD8-Flag) and CRISPR-Cas (Cas13b (C2c4)-dsDNAHD/Cas105' AT-rich PAM-ROSA26) systems.
| Clone | 9B13 |
| Host | Mouse |
| Targeting Cell Type | Ramos (RA 1) |
| Reporter gene | GUS |
| Promoter | hPGK |
| Signal peptides | Sec/SPII |
| Signaling domains | CD79β |
| TM domains | CD8 |
| Detection tags | Flag |
| Linkers | Glycine linker |
| Co-Receptors | FcγRIIB |
| Vector Type | Lentivirus |
| Vector Name | BGpCDH2 |
| Class | 2 single-Cas |
| Type | III |
| Subtype | A, B, C |
| Effector | Cas13b (C2c4) |
| DNA Type | dsDNA |
| Nuclease domains | HD/Cas10 |
| TracrRNA requirement | No |
| PAM/PFS | 5' AT-rich PAM |
| Insertion | ROSA26 |
| Construction | GUS-hPGK-CD79β-Cas13b (C2c4)-dsDNA-ROSA26 |
| Target | S100A4 |
| Alternative Names | S100A4; 18A2; 42A; CAPL; FSP1; MTS1; P9KA; PEL98; S100 calcium binding protein A4 |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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